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Monolayer Techniques for Studying Lipase Kinetics

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Lipase and Phospholipase Protocols

Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 109))

Abstract

Kinetic analyses of lipases and phospholipases must take into account the fact that these enzymes generally catalyze their reactions at a lipid-water interface as opposed to catalysis in a monophasic aqueous environment. This chapter discusses the application of an important tool in the kinetic modeling of these unique enzymes, the monolayer technique. The purpose of this chapter is to give an overview of kinetic modeling as applied to interfacial enzyme lipolysis, a brief discussion of the monolayer technique for studying lipase kinetics, and some applications of the monolayer technique to study substrate composition and stereoselectivity, lipolytic inhibitors, interfacial binding and acylglycerol synthesis.

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Author information

Authors and Affiliations

  1. Laboratoire de Lipolyse Enzymatique, C.N.R.S., Marseille, France

    Stéphane Ransac & Robert Verger

  2. Biophysical Chemistry Laboratory, University of Sofia, Sofia, Bulgaria

    Margarita Ivanova & Ivan Panaiotov

Authors
  1. Stéphane Ransac
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  2. Margarita Ivanova
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  3. Ivan Panaiotov
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  4. Robert Verger
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Editors and Affiliations

  1. University of California and West Los Angeles VA Medical Center, Los Angeles, CA

    Mark Doolittle  & Karen Reue  & 

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© 1999 Humana Press Inc, Totowa, NJ

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Ransac, S., Ivanova, M., Panaiotov, I., Verger, R. (1999). Monolayer Techniques for Studying Lipase Kinetics. In: Doolittle, M., Reue, K. (eds) Lipase and Phospholipase Protocols. Methods in Molecular Biology™, vol 109. Humana Press. https://doi.org/10.1385/1-59259-581-2:279

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  • DOI: https://doi.org/10.1385/1-59259-581-2:279

  • Publisher Name: Humana Press

  • Print ISBN: 978-0-89603-546-1

  • Online ISBN: 978-1-59259-581-5

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