Abstract
The quantitation of amino acids that are present in the acid hydrolysate of proteins and peptides was first developed into an automated procedure by Moore and Stein (1) in the mid-1950s. Quantitation was achieved by hydrindantin hydrate (ninhydrin) derivatization of the amino acids after ion-exchange chromatographic separation. Automated postcolumn amino acid analysis remains largely unchanged today in terms of the basic separation and derivatization chemistry. It is still the “gold standard” to which other methods are compared and is a testimonial to the robust nature of this chemistry.
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References
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© 2003 Humana Press Inc.
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Smith, A.J. (2003). Post Column Amino Acid Analysis. In: Smith, B.J. (eds) Protein Sequencing Protocols. Methods in Molecular Biology™, vol 211. Humana Press. https://doi.org/10.1385/1-59259-342-9:133
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DOI: https://doi.org/10.1385/1-59259-342-9:133
Publisher Name: Humana Press
Print ISBN: 978-0-89603-975-9
Online ISBN: 978-1-59259-342-2
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