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A Stochastic PCR Approach for RNA Quantification in Multiple Samples

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Quantitative PCR Protocols

Part of the book series: Methods in Molecular Medicine™ ((MIMM,volume 26))

Abstract

When studying the effect of various treatments on gene expression in humans, one occasionally is faced with the problem of detecting small changes in transcript levels in minute tissue samples. In addition, interindividual variations can be quite large and may even be the major source of variation (1). Therefore, numerous samples usually have to be analyzed to detect such small variations in gene expression.

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Author information

Authors and Affiliations

  1. Department of Anatomy, University of Bern, Switzerland

    Adrian Puntschart & Michael Vogt

Authors
  1. Adrian Puntschart
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  2. Michael Vogt
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Editor information

Editors and Affiliations

  1. University of Regensburg, Germany

    Bernd Kochanowski  & Udo Reischl  & 

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© 1999 Humana Press Inc., Totowa, NJ

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Puntschart, A., Vogt, M. (1999). A Stochastic PCR Approach for RNA Quantification in Multiple Samples. In: Kochanowski, B., Reischl, U. (eds) Quantitative PCR Protocols. Methods in Molecular Medicine™, vol 26. Humana Press. https://doi.org/10.1385/0-89603-518-2:277

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  • DOI: https://doi.org/10.1385/0-89603-518-2:277

  • Publisher Name: Humana Press

  • Print ISBN: 978-0-89603-518-8

  • Online ISBN: 978-1-59259-262-3

  • eBook Packages: Springer Protocols

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