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Preparation and Use of Tethered Ligands as Biomaterials and Tools for Cell Biology

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Tissue Engineering Methods and Protocols

Part of the book series: Methods in Molecular Medicine™ ((MIMM,volume 18))

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Abstract

The immobilization of biomolecules to various supports has been an important research area for many years. Molecules such as heparin (1,2), as well as various enzymes (3,4), antibodies (5,6), and adhesion ligands (7), have been bound to such supports as silicon or glass, agarose gels, polyethylene oxide (PEO) gels, poly(vinyl alcohol) (PVA) gels, and polymer surfaces. These studies have had important applications in the creation of antithrombogenic surfaces for blood contact, affinity chromatography, and tissue growth and regeneration.

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References

  1. Tay, S. W., Merrill, E. W., Salzman, E. W., and Lindon, J. (1989) Activity towards thrombin-antithrombin of heparin immobilized on two hydrogels. Biomaterials 10, 11–15.

    Article  CAS  Google Scholar 

  2. Lindberg, B., Maripuu, R., Siegbahn, K., Larsson, R., Gölander, C.-G., and Eriksson, J. C. (1983) ESCA studies of heparinized and related surfaces. J. Coll. Int. Sci. 95, 308–321.

    Article  CAS  Google Scholar 

  3. Trevan, M., ed. (1980) Immobilized Enzymes: An Introduction and Applications in Biotechnology, Wiley: New York.

    Google Scholar 

  4. Leckband, D. and Langer, R. (1991) An approach for the stable immobilization of proteins. Biotechnol. Bioeng. 37, 227–237.

    Article  CAS  Google Scholar 

  5. Huang, S.-C., Caldwell, K. D., Lin, J.-N., Wang, H.-K., and Herron, J. N. (1996) Site-specific immobilization of monoclonal antibodies using spacer-mediated antibody attachment. Langmuir 12, 4292–4298.

    Article  CAS  Google Scholar 

  6. von Sommern, A. P. G., Machielsen, P. A. G. M., and Gribnau, T. C. J. (1993) Comparison of three activated agaroses for use in affinity chromatography. J. Chromatog. 639, 23–31.

    Article  Google Scholar 

  7. Massia, S. P. and Hubbell, J. A. (1990) Covalent surface immobilization of Arg-Gly-Asp and Tyr-Ile-Gly-Ser-Arg-containing peptides to obtrain well-defined cell-adhesive substrates. Analyt. Biochem. 187, 292–301.

    Article  CAS  Google Scholar 

  8. Weigel, P. H., Schnaar, R. L., Kuhelnschmidt, M. S., Schmell, E., Lee, R. T., Lee, Y. C., and Roseman, S. (1979) Adhesion of hepatocytes to immobilized sugars: a threshold phenomenon. J. Biol. Chem. 254, 10,830–10,838.

    CAS  Google Scholar 

  9. Massia, S. P. and Hubbell, J. A. (1991) An RGD spacing of 440 nm is sufficient for integrin alpha V beta 3-mediated fibroblast spreading and 140 nm for focal contact and stress fiber formation. J. Cell Biol. 114, 1089–1100.

    Article  CAS  Google Scholar 

  10. Kornberg, L. J., Earp, H. S., Turner, C. E., Prockop, C., and Juliano, R. L. (1991) Signal transduction by integrins: Increased protein tyrosine phosphorylation caused by clustering of beta-1 integrins. Proc. Natl. Acad. Sci. USA 88, 8392–8396.

    Article  CAS  Google Scholar 

  11. Plopper, G. E., McNamee, H. P., Dike, L. E., Bojanowski, K., and Ingber, D. E. (1995) Convergence of integrin and growth factor receptor signaling pathways within the focal adhesion complex. Mol. Biol. Cell 6, 1349–1365.

    CAS  Google Scholar 

  12. Lopina, S. T., Wu, G., Merrill, E. W., and Griffith-Cima, L. G. (1996) Hepatocyte culture on carbohydrate-modified star polyethylene oxide hydrogels. Biomaterials 17, 559–569.

    Article  CAS  Google Scholar 

  13. Sorkin, A. and Carpenter, G. (1991) Dimerization of internalized epidermal growth factor receptors. J. Biol. Chem. 266, 23453–23460.

    CAS  Google Scholar 

  14. Mohammadi, M., Honegger, A., Sorokin, A., Ullrich, A., Schlessinger, J., and Hurwitz, D. R. (1993) Aggregation-induced activation of the epidermal growth factor receptor protein tyrosine kinase. Biochemistry 32, 8742–8748.

    Article  CAS  Google Scholar 

  15. Carraway, K. L. and Cerione, R. A. (1993) Inhibition of growth factor receptor aggregation by an antibody directed against the epidermal growth factor receptor extracellular domain. J. Biol. Chem. 268, 23,860–23,867.

    CAS  Google Scholar 

  16. Merrill, E. W. (1993) Poly(ethylene oxide) star molecules: synthesis characterization, and applications in medicine and biology. J. Biomater. Sci., Polymer Ed., in press.

    Google Scholar 

  17. Gnanou, Y., Lutz, P., and Rempp, P. (1988) Synthesis of star-shaped poly(ethylene oxide). Makromol. Chemie 189, 2885–2892.

    Article  CAS  Google Scholar 

  18. Lutz, P. and Rempp, P. (1988) New developments in star polymer synthesis: star-shaped polystyrenes and star-block copolymers. Makromol. Chemei 189, 1051–1060.

    Article  CAS  Google Scholar 

  19. Wong, J. Y., Kuhl, T. L., Israelachvili, J. N., Mullah, N., and Zalipsky, S. (1997) Direct measure of a tethered ligand-receptor interaction potential. Science 275, 820–823.

    Article  CAS  Google Scholar 

  20. Harris, J. M., ed. (1992) Poly(ethylene glycol) Chemistry. Topics in Applied Chemistry (Katritzky, A. R. and Sabongi, G. J., eds.), Plenum, New York, p. 385.

    Google Scholar 

  21. Forni, A., Ganazzoli, F., and Vacatello, M. (1996) Local conformation of regular star polymers in a good solvent: a Monte Carlo study. Macromolecules 29, 2994–2999.

    Article  CAS  Google Scholar 

  22. Grest, G. S., Kremer, K., and Witten, T. A. (1987) Structure of many-arm star polymers: a molecular dynamics simulation. Macromol. 20, 1376–1383.

    Article  CAS  Google Scholar 

  23. Kuhl, P. R. and Griffith-Cima, L. G. (1996) Tethered epidermal growth factor as a paradigm for growth factor-induced stimulation from the solid phase. Nature Med. 2, 1022–1027.

    Article  CAS  Google Scholar 

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Authors and Affiliations

  1. Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, MA

    Susan J. Sofia, Philip R. Kuhl & Linda G. Griffith

Authors
  1. Susan J. Sofia
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  2. Philip R. Kuhl
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  3. Linda G. Griffith
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Editor information

Editors and Affiliations

  1. Center for Engineering in Medicine, Massachusetts General Hospital/Harvard Medical School, Boston, MA

    Jeffrey R. Morgan PhD  & Martin L. Yarmush MD  & 

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© 1999 Humana Press Inc., Totowa, NJ

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Sofia, S.J., Kuhl, P.R., Griffith, L.G. (1999). Preparation and Use of Tethered Ligands as Biomaterials and Tools for Cell Biology. In: Morgan, J.R., Yarmush, M.L. (eds) Tissue Engineering Methods and Protocols. Methods in Molecular Medicine™, vol 18. Humana Press. https://doi.org/10.1385/0-89603-516-6:19

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  • DOI: https://doi.org/10.1385/0-89603-516-6:19

  • Publisher Name: Humana Press

  • Print ISBN: 978-0-89603-516-4

  • Online ISBN: 978-1-59259-602-7

  • eBook Packages: Springer Protocols

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