Abstract
Terminal deoxyribonucleotidyl transferase (TdT) (EC 2.7.7.3 1) catalyzes the addition of deoxynucleoside triphosphates to the 3′ ends of oligo-and polynucleotide primers. Different from all the other known DNA polymerases, TdT-catalyzed DNA synthesis is not template-directed. The reaction can be described as:
Only one dNTP is necessary for polymerization, although any dNTP (and derivatives thereof) or any combination of dNTPs will serve as substrate.
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Grosse, F., Manns, A. (1993). Terminal Deoxyribonucleotidyl Transferase (EC 2.7.7.31). In: Burrell, M.M. (eds) Enzymes of Molecular Biology. Methods in Molecular Biology™, vol 16. Humana Press. https://doi.org/10.1385/0-89603-234-5:95
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DOI: https://doi.org/10.1385/0-89603-234-5:95
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