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Avidin–Biotin Technology

Preparation of Biotinylated Probes

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Immunochemical Protocols

Part of the book series: Methods in Molecular Biology ((MIMB,volume 10))

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Abstract

During the past decade, the avidin-biotin complex has become a useful and versatile tool for application in a variety of biological disciplines (1–3). One of the major applications is in immunology and related areas (4).

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References

  1. Bayer, E. A. and Wilchek, M. (1978) The avidin-biotin complex as a tool in molecular biology. Trends Biochem. Sci. 3, N237–N239.

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  2. Bayer, E. A. and Wilchek, M. (1980) The use of the avidin-biotin complex in molecular biology. Methods Biochem. Anal. 26, 1–45.

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  3. Wilchek, M. and Bayer, E. A., eds. (1990) Avidin-Biotin Technology. Methods in Enzymology, vol. 184, Academic, Orlando, FL, pp. 3–746.

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  4. Wilchek, M. and Bayer, E. A. (1984) The avidin-biotin complex in immunology. Immunol Today 5, 3943.

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  5. Wilchek, M. and Bayer, E. A. (1989) Avidin-biotin technology ten years on: Has it lived up to its expectations? Trends Biochem. Sci. 14, 408–412.

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  6. Wilchek, M. and Bayer, E. A. (1988) The avidin-biotin complex in bioanalytical applications. Anal. Biochem. 171, 1–32.

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Author information

Authors and Affiliations

  1. Department of Biophysics, The Weizmann Institute of Science, Rehovot, Israel

    Edward A. Bayer & Meir Wilchek

Authors
  1. Edward A. Bayer
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  2. Meir Wilchek
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Editor information

Editors and Affiliations

  1. Medical Research Council Laboratories, Carshalton, Surrey, UK

    Margaret M. Manson

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© 1992 The Humana Press, Inc., Totowa, NJ

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Bayer, E.A., Wilchek, M. (1992). Avidin–Biotin Technology. In: Manson, M.M. (eds) Immunochemical Protocols. Methods in Molecular Biology, vol 10. Humana Press, Totowa, NJ. https://doi.org/10.1385/0-89603-204-3:137

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  • DOI: https://doi.org/10.1385/0-89603-204-3:137

  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-0-89603-204-0

  • Online ISBN: 978-1-59259-497-9

  • eBook Packages: Springer Protocols

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