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The Bradford Method for Protein Quantitation

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New Protein Techniques

Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 3))

Abstract

A rapid and accurate method for the estimation of protein concentration is essential in many fields of protein study. The Lowry method (Chapter 1 in vol. 1 of this series) has been widely used, but is susceptible to interference from a wide range of compounds commonly present in biological extracts. Although interference can be avoided by trichloracetic acid precipitation of the protein prior to assay, this lengthens the procedure.

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References

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© 1988 The Humana Press Inc.

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Hammond, J.B.W., Kruger, N.J. (1988). The Bradford Method for Protein Quantitation. In: Walker, J.M. (eds) New Protein Techniques. Methods in Molecular Biology™, vol 3. Humana Press. https://doi.org/10.1385/0-89603-126-8:25

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  • DOI: https://doi.org/10.1385/0-89603-126-8:25

  • Publisher Name: Humana Press

  • Print ISBN: 978-0-89603-126-5

  • Online ISBN: 978-1-59259-490-0

  • eBook Packages: Springer Protocols

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