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Federica Finetti, Erika Terzuoli, Sandra Donnini, Marianna Uva, Marina Ziche, Lucia Morbidelli
Monitoring Endothelial and Tissue Responses to Cobalt Ferrite Nanoparticles and Hybrid Hydrogels
www.plosone.org
www.plosone.org
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1 TL
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q
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Q
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7.4999 0 0 6.6659 367.7102 608.1448 cm
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1 TL
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q
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1 J
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7.4999 0 0 6.6659 415.7291 608.1448 cm
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1 TL
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q
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1 J
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BT
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1 TL
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ET
Q
q
1 j
1 J
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7.4999 0 0 6.6659 426.7275 608.1448 cm
BT
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1 TL
-0.0053 Tc
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ET
Q
q
1 j
1 J
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10 0 0 10 430.2992 609.7889 cm
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Q
q
1 j
1 J
0 w
7.4999 0 0 6.6659 448.8377 608.1448 cm
BT
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1 TL
-0.0053 Tc
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(2)Tj
ET
Q
q
1 j
1 J
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1 TL
-0.004 Tc
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(NPs,)Tj
2.0976 0 Td
(CMC)Tj
2.4378 0 Td
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Q
q
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7.4999 0 0 6.6659 229.2094 595.1622 cm
BT
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1 TL
-0.0053 Tc
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ET
Q
q
1 j
1 J
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10 0 0 10 232.7811 596.8062 cm
BT
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1 TL
-0.004 Tc
0 0 Td
(O)Tj
ET
Q
q
1 j
1 J
0 w
7.4999 0 0 6.6659 240.2645 595.1622 cm
BT
/F6 1 Tf
1 TL
-0.0053 Tc
0 0 Td
(4)Tj
ET
Q
q
1 j
1 J
0 w
10 0 0 10 245.9905 596.8062 cm
BT
/F6 1 Tf
1 TL
-0.004 Tc
0 0 Td
(NPs)Tj
1.8765 0 Td
(or)Tj
1.0998 0 Td
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ET
Q
q
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1 J
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7.4999 0 0 6.6659 296.9574 595.1622 cm
BT
/F6 1 Tf
1 TL
-0.0053 Tc
0 0 Td
(2)Tj
ET
Q
q
1 j
1 J
0 w
10 0 0 10 300.5291 596.8062 cm
BT
/F6 1 Tf
1 TL
-0.004 Tc
0 0 Td
(O)Tj
ET
Q
q
1 j
1 J
0 w
7.4999 0 0 6.6659 307.9559 595.1622 cm
BT
/F6 1 Tf
1 TL
-0.0053 Tc
0 0 Td
(4)Tj
ET
Q
q
1 j
1 J
0 w
10 0 0 10 311.5275 596.8062 cm
BT
/F6 1 Tf
1 TL
-0.004 Tc
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(-NH)Tj
ET
Q
q
1 j
1 J
0 w
7.4999 0 0 6.6659 330.0661 595.1622 cm
BT
/F6 1 Tf
1 TL
-0.0053 Tc
0 0 Td
(2)Tj
ET
Q
q
1 j
1 J
0 w
10 0 0 10 335.8488 596.8062 cm
BT
/F6 1 Tf
1 TL
-0.004 Tc
0 0 Td
(NPs])Tj
2.2166 0 Td
(were)Tj
2.1203 0 Td
(sterilized)Tj
3.8211 0 Td
(by)Tj
1.1792 0 Td
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(\(20)Tj
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0.9525 0 Td
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Q
q
1 j
1 J
0 w
7.4999 0 0 6.6659 355.0677 588.2456 cm
BT
/F6 1 Tf
1 TL
-0.0053 Tc
0 0 Td
(4)Tj
ET
Q
q
1 j
1 J
0 w
10 0 0 10 360.7937 583.8236 cm
BT
/F6 1 Tf
1 TL
-0.004 Tc
0 0 Td
(on)Tj
1.2755 0 Td
(immunofluorescence)Tj
8.6797 0 Td
(coverslips)Tj
4.1499 0 Td
(located)Tj
3.0784 0 Td
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1.0374 0 Td
(24)Tj
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0.9751 0 Td
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(or)Tj
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(gels)Tj
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1.0262 0 Td
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0.9524 0 Td
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1.8709 0 Td
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0.6917 0 Td
(days)Tj
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4.7735 0 Td
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1.9955 0 Td
(100%)Tj
2.4038 0 Td
(methanol)Tj
4.0251 0 Td
(and)Tj
1.7292 0 Td
(stained)Tj
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(with)Tj
2.0012 0 Td
(hematoxylin)Tj
5.2101 0 Td
(and)Tj
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(eosin)Tj
2.3244 0 Td
(\(H&E\))Tj
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1.0261 0 Td
(monitor)Tj
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(number.)Tj
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(and)Tj
1.7291 0 Td
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3.5887 0 Td
(cells)Tj
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1.0374 0 Td
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1.1679 0 Td
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Q
q
1 j
1 J
0 w
11.9999 0 0 11.9999 200.0125 490.2236 cm
BT
/F0 1 Tf
1 TL
-0.0033 Tc
0 0 Td
(MTT)Tj
2.2677 0 Td
(assay)Tj
ET
Q
q
1 j
1 J
0 w
10 0 0 10 200.0125 473.2157 cm
BT
/F6 1 Tf
1 TL
-0.004 Tc
0 0 Td
(HUVEC)Tj
3.651 0 Td
(were)Tj
2.1203 0 Td
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0.9524 0 Td
(the)Tj
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3.101 0 Td
(of)Tj
1.0148 0 Td
(1500/well)Tj
4.0535 0 Td
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1.0375 0 Td
(96)Tj
1.1679 0 Td
(multiwell)Tj
3.9798 0 Td
(plates)Tj
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(or)Tj
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(68)Tj
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(h,)Tj
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(medium)Tj
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(4)Tj
0.6973 0 Td
(h)Tj
0.7483 0 Td
(with)Tj
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(fresh)Tj
2.194 0 Td
(medium)Tj
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(presence)Tj
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10.9246 0 Td
(l\)-2,5-diphenyltetrazolium)Tj
10.7999 0 Td
(bromide\))Tj
3.9855 0 Td
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2.8347 0 Td
(Italy\).)Tj
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2.3358 0 Td
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1.0261 0 Td
(a)Tj
0.652 0 Td
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3.4752 0 Td
(pigmented)Tj
4.5071 0 Td
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4.0365 0 Td
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1.0375 0 Td
(DMSO,)Tj
-33.3749 -1.3039 Td
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4.7338 0 Td
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1.0148 0 Td
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1.4683 0 Td
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4.0365 0 Td
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4.5297 0 Td
(reader)Tj
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2.8857 0 Td
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-35.807 -1.2982 Td
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2.0636 0 Td
(CA,)Tj
1.7914 0 Td
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0.9524 0 Td
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1.6441 0 Td
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4.0932 0 Td
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1.0148 0 Td
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4.7395 0 Td
(units)Tj
2.2224 0 Td
(\(Abs\))Tj
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(20)Tj
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Q
q
1 j
1 J
0 w
11.9999 0 0 11.9999 200.0125 366.5763 cm
BT
/F0 1 Tf
1 TL
-0.0033 Tc
0 0 Td
(Western)Tj
3.9118 0 Td
(blot)Tj
ET
Q
q
1 j
1 J
0 w
10 0 0 10 200.0125 349.6251 cm
BT
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1 TL
-0.004 Tc
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4.8131 0 Td
(cells)Tj
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1.1736 0 Td
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(10%)Tj
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2.948 0 Td
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2.2847 0 Td
(cell)Tj
1.5647 0 Td
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4.0365 0 Td
(NPs)Tj
1.8765 0 Td
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1.0999 0 Td
(lyophilized)Tj
4.6204 0 Td
(biomaterials)Tj
5.1534 0 Td
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(dissolved)Tj
3.9004 0 Td
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-33.5279 -1.2982 Td
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1.7291 0 Td
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2.7893 0 Td
(in)Tj
1.0375 0 Td
(direct)Tj
2.5341 0 Td
(contact)Tj
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(24)Tj
1.1735 0 Td
(h)Tj
0.7484 0 Td
(incubation,)Tj
4.7735 0 Td
(biomaterials)Tj
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3.7473 0 Td
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1.7348 0 Td
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4.0478 0 Td
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1.0375 0 Td
([)Tj
0.83 0.64 0.02 0 k
(20)Tj
0 g
(].)Tj
2.075 0 Td
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1.8028 0 Td
(lysates)Tj
2.7949 0 Td
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(10000)Tj
-35.0473 -1.3039 Td
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(20)Tj
1.1736 0 Td
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1.8538 0 Td
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(4C.)Tj
1.9786 0 Td
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4.2179 0 Td
(gel)Tj
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(electrophoresis,)Tj
6.4743 0 Td
(proteins)Tj
3.5149 0 Td
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(blotted)Tj
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(activated)Tj
3.7814 0 Td
(nitro-)Tj
-34.5994 -1.2983 Td
(cellulose)Tj
3.6226 0 Td
(membranes,)Tj
5.1534 0 Td
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(overnight)Tj
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(the)Tj
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(anti)Tj
1.7689 0 Td
(caspase-3)Tj
3.9968 0 Td
(antibody)Tj
3.7644 0 Td
(\(1:1000\))Tj
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(Sig-)Tj
-35.6822 -1.2982 Td
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dup 7 /C7 put
dup 8 /C8 put
dup 9 /C9 put
dup 10 /C10 put
dup 11 /C11 put
dup 12 /C12 put
dup 13 /C13 put
dup 14 /C14 put
dup 15 /C15 put
dup 16 /C16 put
dup 17 /C17 put
dup 18 /C18 put
dup 19 /C19 put
dup 20 /C20 put
dup 21 /C21 put
dup 22 /C22 put
dup 23 /C23 put
dup 24 /C24 put
dup 25 /C25 put
dup 26 /C26 put
dup 27 /C27 put
dup 28 /C28 put
dup 29 /C29 put
dup 30 /C30 put
dup 31 /C31 put
dup 32 /space put
dup 33 /exclam put
dup 34 /quotedbl put
dup 35 /numbersign put
dup 36 /dollar put
dup 37 /percent put
dup 38 /ampersand put
dup 39 /quoteright put
dup 40 /parenleft put
dup 41 /parenright put
dup 42 /asterisk put
dup 43 /plus put
dup 44 /comma put
dup 45 /hyphen put
dup 46 /period put
dup 47 /slash put
dup 48 /zero put
dup 49 /one put
dup 50 /two put
dup 51 /three put
dup 52 /four put
dup 53 /five put
dup 54 /six put
dup 55 /seven put
dup 56 /eight put
dup 57 /nine put
dup 58 /colon put
dup 59 /semicolon put
dup 60 /less put
dup 61 /equal put
dup 62 /greater put
dup 63 /question put
dup 64 /at put
dup 65 /A put
dup 66 /B put
dup 67 /C put
dup 68 /D put
dup 69 /E put
dup 70 /F put
dup 71 /G put
dup 72 /H put
dup 73 /I put
dup 74 /J put
dup 75 /K put
dup 76 /L put
dup 77 /M put
dup 78 /N put
dup 79 /O put
dup 80 /P put
dup 81 /Q put
dup 82 /R put
dup 83 /S put
dup 84 /T put
dup 85 /U put
dup 86 /V put
dup 87 /W put
dup 88 /X put
dup 89 /Y put
dup 90 /Z put
dup 91 /bracketleft put
dup 92 /backslash put
dup 93 /bracketright put
dup 94 /asciicircum put
dup 95 /underscore put
dup 96 /quoteleft put
dup 97 /a put
dup 98 /b put
dup 99 /c put
dup 100 /d put
dup 101 /e put
dup 102 /f put
dup 103 /g put
dup 104 /h put
dup 105 /i put
dup 106 /j put
dup 107 /k put
dup 108 /l put
dup 109 /m put
dup 110 /n put
dup 111 /o put
dup 112 /p put
dup 113 /q put
dup 114 /r put
dup 115 /s put
dup 116 /t put
dup 117 /u put
dup 118 /v put
dup 119 /w put
dup 120 /x put
dup 121 /y put
dup 122 /z put
dup 123 /braceleft put
dup 124 /bar put
dup 125 /braceright put
dup 126 /asciitilde put
dup 127 /Euro put
readonly def
currentdict end
currentfile eexec
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