DCA1 Acts as a Transcriptional Co-activator of DST and Contributes to Drought and Salt Tolerance in Rice
Fig 1
Physical interaction between DCA1 and DST.
(A) Yeast two-hybrid constructs using native and variant DST and DCA1 fragments. (B) Growth of yeast cells harboring various combinations of constructs. SD/-Leu/-Trp and SD/-Ade/-His/-Leu/-Trp were used as nonselective and selective media, respectively. The assay revealed an interaction between DCA1 and both full-length DST and the C-terminal fragment of DST (△N 1–72 DST). (C) BiFC assay in Arabidopsis protoplasts revealing the interaction between DST and DCA1 in vivo. Y, YFP; BF, Bright Field; Chl, Chlorophyll; M, Merge. (D) In vitro pull-down of His2-tagged DCA1 using MBP-tagged DST as determined by immunoblotting with anti-MBP antibody (left) and anti-His antibody (right).