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Quantitative Determination of Allicin in Allium sativum L. Bulbs by UPLC

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Abstract

An UPLC method for determination of allicin in garlic bulb powder has been developed and validated. Chromatographic separation and determination were performed on an Acquity UPLC BEH C18 column using a mobile phase of methanol/water (50:50, v/v). Linearity between the allicin concentration and the peak area was good within the concentration ranges 2.04–510 mg L−1. The method was validated over this range for both accuracy and precision. The method was successfully used for the quantitative analysis of allicin in ten garlic varieties.

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Acknowledgment

China National Science and Technology Infrastructure Fund(2005DKA21002-33) and China National R&D Special Fund for Public Welfare Industry(200903018-03) supported this research.

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Authors and Affiliations

  1. Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, 12 Nandajie, Zhongguancun, Haidian District, 100081, Beijing, China

    Haiping Wang, Xixiang Li, Su Liu & Song Jin

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  1. Haiping Wang
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  2. Xixiang Li
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  3. Su Liu
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  4. Song Jin
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Correspondence to Xixiang Li.

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Wang, H., Li, X., Liu, S. et al. Quantitative Determination of Allicin in Allium sativum L. Bulbs by UPLC. Chroma 71, 159–161 (2010). https://doi.org/10.1365/s10337-009-1416-1

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  • DOI: https://doi.org/10.1365/s10337-009-1416-1

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