Abstract
Turbulent flow chromatography coupled to tandem mass spectrometry (TFC-MS-MS) has been widely used within bioanalysis, because of the ability to inject directly neat biological samples with no prior pretreatment. TFC-MS-MS removes the need for time consuming sample preparation procedures such as protein precipitation, liquid-liquid extraction or solid-phase extraction. There are two standard configurations that are commonly adopted for the use of TFC, namely fast elute and focus mode. In this paper, a new micro TFC column which has the advantages of reducing solvent consumption and improving mass sensitivity, was used to develop a fast elute method. A wide range of pharmaceutical compounds with various physicochemical properties was used to assess the system performance. Carry-over has often been identified as a potential source of inaccuracy in a fast elute mode. This paper shows how by using a systematic approach, carryover was eliminated. The parameters influencing the robustness of the micro TFC column are also discussed. This method was fully validated for a Pfizer development compound in human plasma using a new TFC parallel platform allowing two systems to be operated in parallel. Multiplexing the sample analysis allowed a 2-fold increase in throughput and provided an efficient use of the mass spectrometer.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Quinn HM, Takarewski JJ (1997) Int Patent Number WO 97/16724
Edge T (2003) Turbulent flow chromatography in bioanalysis. Handbook of Analytical Separations (2003), 4 (Bioanalytical Separations), 91–128
Edge T (2002) The application of turbulent flow liquid chromatography to high speed analysis. CAST, Chromatography and Separation Technology 23(10–14):16
Rudewicz PJ, Yang L (2001) Am Pharm Review 4(2):64,6668,70
Walter RE, Cramer JA, Tse FLS (2001) J Pharm Biomed Anal 25(2):331–337
Zimmer D, Pickard V, Czembor W, Müller C (1999) J Chromatogr A 854(1+2):23–35
Hopfgartner G, Husser C, Zell M (2002) Therap Drug Monitoring 24(1):134–143
Ynddal L, Hansen SH (2003) J Chromatogr A 1020(1):59–67
Krebber R (2003) J Vet Pharm Therapeutics (Suppl 1) 26:102–103
Takino M, Daishima S, Yamaguchi K, Nakahara T (2003) Analyst 128 (1):46–50
Brignol N, Bakhtiar R, Dou L, Majumdar T, Tse FLS (2000) Rapid Commun. Mass Spectrom 14(3):141–149
Ramos L, Brignol N, Bakhtiar R, Ray T, McMahon LM, Tse FLS (2000) Rapid Commun Mass Spectrom 14(23):2282–2293
Jemal M, Xia YQ, Whigan DB (1998) Rapid Commun, Mass Spectrom 12:1389
Long JM, James CA, Clarke BJ, Castelli MG, Rolando S (2002) Chromatogr (Suppl) 55:S31–S34
Ayrton J, Dear GJ, Leavens WJ, Mallet DN, Plumb RS (1997) Rapid Commun Mass Spectrom 11(18):1953–1958
Herman JL (2002) Rapid Commun Mass Spectrom 16(5):421–426
Wu JT, Zeng H, Qian M, Brogdon BL, Unger SE (2000) Anal Chem 72:61
Lim HK, Chan KW, Sisenwine S, Scatina JA (2001) Anal Chem 73(9):2140–2146
Chassaing C, Luckwell J, Macrae P, Saunders K, Wright P, Venn R (2001) Chromatographia 53:122
Koal T, Asperger A, Efer J, Engewald W (2003) Chromatogr 57:93–101
Ayrton J, Dear GJ, Leavens WJ, Mallet DN, Plumb RS (1999) Rapid Commun Mass Spectrom 13:1657
Grant RP, Cameron C, Mackenzie-McMurter S (2002) Rapid Commun Mass Spectrom 16(18):1785–1792
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Chassaing, C., Stafford, H., Luckwell, J. et al. A Parallel Micro Turbulent Flow Chromatography-Tandem Mass Spectrometry Method for the Analysis of a Pharmaceutical Compound in Plasma. Chroma 62, 17–24 (2005). https://doi.org/10.1365/s10337-005-0562-3
Received:
Revised:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1365/s10337-005-0562-3