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Augmented Therapeutic Efficacy of Naringenin Through Microemulsion-Loaded Sericin Gel Against UVB-Induced Photoaging

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Abstract

Naringenin (NAR) is a flavonoid found in citrus fruits such as grapes and oranges. Recently, NAR has demonstrated its potential in inhibition of photoaging. The aim of the present study was to investigate the efficacy of sericin (SR) gel loaded with NAR microemulsion (ME) to inhibit UVB-induced photoaging and prevention of epidermoid carcinoma in animal model. NAR -ME was prepared and optimized through Box–Behnken design. The optimized ME was loaded into sericin (SR) gel. The formulations were subjected to various in vitro, in vivo and cytotoxicity studies over A431 cell lines. The optimized ME revealed a globule size of 249.05 ± 3.78 nm, 6.7 ± 0.5 pH and 73.1 ± 2.11% release over a period of 24 h respectively. Cytotoxicity studies revealed a depression in IC50 value in NAR -ME (65.11 ± 1.54 μg/ml) when compared with NAR (118.1 ± 2.09 μg/ml). The NAR-ME-SR gel displayed enhanced therapeutic potential when compared with plain NAR, in terms of augmented antiproliferative activity.

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Abbreviations

BBD:

Box–Behnken design

FACS:

Fluorescence-activated cell sorting

ICH:

International Conference on Harmonization

ME:

Microemulsion

MMP:

Mitochondrial membrane potential

MTT:

Methylthiazoltetrazolium

mV:

Milli volt

Nar:

Naringenin

nm:

Nanometre

OA:

Oleic acid

ROS:

Reactive oxygen species

RPMI:

Roswell Park Memorial Institute medium

UV:

Ultra violet

WGO:

Wheat germ oil

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Authors and Affiliations

  1. Department of Pharmaceutical Sciences, Babasaheb Bhimrao Ambedkar University (A Central University), Vidya Vihar, Raebareli Road, Lucknow, 226025, India

    Poonam Parashar, Surabhi Pal, Monika Dwivedi & Shubhini A. Saraf

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  1. Poonam Parashar
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  2. Surabhi Pal
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  4. Shubhini A. Saraf
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Correspondence to Shubhini A. Saraf.

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Parashar, P., Pal, S., Dwivedi, M. et al. Augmented Therapeutic Efficacy of Naringenin Through Microemulsion-Loaded Sericin Gel Against UVB-Induced Photoaging. AAPS PharmSciTech 21, 215 (2020). https://doi.org/10.1208/s12249-020-01766-1

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