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Characteristics of Changes in Lipid Peroxidation and Na+/K+-ATPase Activity in the Cortex of Old Rats in Conditions of Two-Vessel Cerebral Ischemia/Reperfusion

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Abstract

The success of studies on the neuroprotective properties of compounds depends on the used model of diseases of the central nervous system on animals. The approaches widely used to model cerebral ischemia/reperfusion were developed on young animals and may not be as efficient or may not be suitable at all for modeling on old animals, which are recommended for preclinical trials. The goal of the study was to detect age-related features of the effect of cerebral reperfusion of different duration (1 and 3 h) after two-vessel forebrain ischemia on the level of lipid peroxidation (LPO) products and the activity of Na+/K+-ATPase in the cerebral cortex of rats aged 22–24 months. We found a late accumulation (3 h, but not 1 h, after restoration of the blood flow) of LPO products, specifically, triene conjugates and Schiff bases, and a decrease in Na+/K+-ATPase activity in the cerebral cortex of old rats as compared with young animals. This indicates the features of the molecular and physiological mechanisms of the development of disorders in the brain during ischemia/reperfusion in old and young animals. It is necessary to take into account the identified differences in these mechanisms in the development and testing of compounds that will be subsequently used for the treatment of elderly patients with stroke and ischemic brain damage.

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Funding

This work was supported by the Russian Foundation for Basic Research (project no. 18-315-00285) and partially by the state tasks of the Federal Agency for Scientific Organizations of Russia (topic no. AAAA-A18-118012290427-7).

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Authors and Affiliations

  1. Sechenov Institute of Evolutionary Physiology and Biochemistry, Russian Academy of Sciences, 194223, St. Petersburg, Russia

    I. I. Zorina, E. A. Fokina, I. O. Zakharova, L. V. Bayunova & A. O. Shpakov

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  1. I. I. Zorina
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  2. E. A. Fokina
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  3. I. O. Zakharova
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  4. L. V. Bayunova
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Correspondence to I. I. Zorina.

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Statement on animal welfare. All experiments were conducted in strict accordance with the regulations developed and approved by the Ethics Committee of the Sechenov Institute of Evolutionary Physiology and Biochemistry, Russian Academy of Sciences, regulations and requirements set out in the documents “European Communities Council Directive 1986” (86/609/EEC) and “Guide for the Care and Use of Laboratory Animals.”

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Translated by A. Barkhash

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Zorina, I.I., Fokina, E.A., Zakharova, I.O. et al. Characteristics of Changes in Lipid Peroxidation and Na+/K+-ATPase Activity in the Cortex of Old Rats in Conditions of Two-Vessel Cerebral Ischemia/Reperfusion. Adv Gerontol 10, 156–161 (2020). https://doi.org/10.1134/S2079057020020162

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