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Comparison of Impregnated Bone Morphogenetic Protein-2 Release Kinetics from Biopolymer Scaffolds

  • MATERIALS FOR ENSURING HUMAN VITAL ACTIVITY AND ENVIRONMENTAL PROTECTION
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Inorganic Materials: Applied Research Aims and scope

Abstract

The purpose of this study was to evaluate the release kinetics of impregnated recombinant human bone morphogenetic protein-2 (rhBMP-2) from different engineered scaffolds. Poly(lactide-co-glycolide) (PLG) matrices prepared by supercritical fluid technologies (SCFT) showed the highest biocompatibility and long-term release of rhBMP-2. There was an even release of rhBMP-2 from them for 11 days. The subsequent use of laser sintering allowed delaying the peak of the protein release for a period of 13 to 15 days. The average loss of rhBMP-2 using SCFT did not exceed 20%. The maximum release of rhBMP-2 from a collagen-fibronectin hydrogel was in the period from 4 to 6 days. But 47 ± 12% rhBMP-2 loss was shown. Highly porous polylactide-based scaffolds obtained by freeze-drying were inferior to other scaffolds in their ability to release rhBMP-2 for a prolonged period. The hydrogel and chitosan-based granules showed high cytotoxicity and a short period of protein release.

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Funding

This study was supported by a grant from the Russian Science Foundation (project no. 16-15-00298) in terms of research on materials based on chitosan and polylactide granules obtained by cryolyophilization, as well as by the Ministry of Science and Higher Education in the framework of the work on the state task of the Federal Research Center Crystallography and Photonics, Russian Academy of Sciences, in terms of the formation of polylactoglycolide matrices by the methods of supercritical fluid plasticization and selective laser sintering and according to the state task of the Research Centre for Medical Genetics for in vitro studies of collagen-fibronectin hydrogel.

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Authors and Affiliations

  1. Central Research Institute of Dentistry and Maxillofacial Surgery, 119021, Moscow, Russia

    A. V. Vasilyev, V. S. Kuznetsova, I. I. Babichenko & A. A. Kulakov

  2. Research Centre for Medical Genetics, 115522, Moscow, Russia

    T. B. Bukharova, E. V. Galitsyna & D. V. Goldshtein

  3. National Research Center Kurchatov Institute, 123182, Moscow, Russia

    Yu. D. Zagoskin, T. E. Grigoriev & S. N. Chvalun

  4. Federal Scientific Research Centre Crystallography and Photonics, Russian Academy of Sciences, 142190, Moscow, Troitsk, Russia

    S. A. Minaeva, E. N. Antonov & V. K. Popov

  5. Imtek Company Ltd., Moscow, Russia

    E. O. Osidak

  6. Cardiology Research and Production Center, Ministry of Health of the Russian Federation, Moscow, Russia

    S. P. Domogatsky

Authors
  1. A. V. Vasilyev
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  2. T. B. Bukharova
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  3. V. S. Kuznetsova
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  4. Yu. D. Zagoskin
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  5. S. A. Minaeva
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  6. T. E. Grigoriev
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  7. E. N. Antonov
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  8. E. O. Osidak
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  9. E. V. Galitsyna
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  10. I. I. Babichenko
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  11. S. P. Domogatsky
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  12. V. K. Popov
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  13. S. N. Chvalun
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  14. D. V. Goldshtein
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  15. A. A. Kulakov
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Correspondence to A. V. Vasilyev.

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Translated by M. Aladina

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Vasilyev, A.V., Bukharova, T.B., Kuznetsova, V.S. et al. Comparison of Impregnated Bone Morphogenetic Protein-2 Release Kinetics from Biopolymer Scaffolds. Inorg. Mater. Appl. Res. 10, 1093–1100 (2019). https://doi.org/10.1134/S2075113319050332

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  • DOI: https://doi.org/10.1134/S2075113319050332

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