Abstract—
Highly porous three-dimensional (3D) cylindrical matrices with a diameter of 1.3 mm were obtained by lyophilization of the chitosan solution. The matrix was implanted into rat muscle tissue and after12 months underwent complete resorption. Cellular mechanisms of matrix bioresorption were examined by histological and immunohistochemical methods in vivo after1, 2, 6, 12, 24, 36, and 48 weeks. The role of CD68+ cells, mainly giant multinucleated of foreign body cells, has been investigated. Chronic aseptic inflammation is not accompanied by the activation of mast cells. It shows the bioinertness of the matrix material and allows us to recommend this matrix for use in regenerative medicine, tissue engineering, and cell transplantology.
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This work was supported by the Russian Science Foundation, project no. 19-73-30003.
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Statement on the welfare of animals. Animal tests were carried out in accordance with the rules for the use of experimental animals (according to the principles of the European Convention, Strasbourg, 1986, and the Helsinki Declaration of the World Medical Association on the Humane Treatment of Animals, 1996).
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Translated by I. Fridlyanskaya
Abbreviations: PCM— porous chitosan matrix; GMCFB—giant multinucleated cell of foreign body; MCs-mast cells.
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Popryadukhin, P.V., Yukina, G.Y., Dobrovolskaya, I.P. et al. Cellular Mechanisms of Bioresorption of a Porous 3D Matrix Based on Chitosan. Cell Tiss. Biol. 13, 463–469 (2019). https://doi.org/10.1134/S1990519X19060087
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DOI: https://doi.org/10.1134/S1990519X19060087