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Apoptosis and necrosis induced by ultraviolet radiation in the presence of autologous plasma

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Abstract

This paper deals with the effect of ultraviolet (UV) light (240–390 nm) at doses of 151–3020 J/m2* on the character of the death of donors’ blood lymphocytic cells (with the use of markers of apoptotic and necrotic cell death) and on the level of the CD95 receptor—a marker of apoptotic predisposition. UV irradiation has been shown to increase the expression of CD95 receptors, which is due mainly to synthesis de novo. It is revealed that over the course of the 24-h incubation of photomodified lymphocytes (at irradiation doses of 151 and 755 J/m2) without autologous blood plasma, cell death occurs by receptor-mediated apoptosis. Action of high irradiation doses (1510 and 3020 J/m2) leads to the massive necrotic death of immunocytes. The use of autologous blood plasma during the incubation of photomodified lymphocytes allows a reduction in the amount of both apoptotic and necrotic cells.

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Abbreviations

ROS:

reactive oxygen species

LPO:

lipid peroxidation

SOD:

superoxidedismutase

UV:

ultraviolet

PI:

propidium iodide

PC5:

complex of phycoerythrin with cyanide 5

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Authors and Affiliations

  1. Department of Biophysics and Biotechnology, Voronezh State University, Voronezh, Russia

    V. G. Artyukhov & O. V. Basharina

  2. Burdenko Voronezh Medical Academy, Voronezh, Russia

    O. V. Zemchenkova, S. V. Ryazantsev & M. V. Pashkov

Authors
  1. V. G. Artyukhov
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  2. O. V. Zemchenkova
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  3. O. V. Basharina
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  4. S. V. Ryazantsev
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  5. M. V. Pashkov
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Corresponding author

Correspondence to O. V. Zemchenkova.

Additional information

Original Russian Text © V.G. Artyukhov, O.V. Zemchenkova, O.V. Basharina, S.V. Ryazantsev, M.V. Pashkov, 2014, published in Tsitologiya, 2014, Vol. 56, No. 1, pp. 77–83.

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Artyukhov, V.G., Zemchenkova, O.V., Basharina, O.V. et al. Apoptosis and necrosis induced by ultraviolet radiation in the presence of autologous plasma. Cell Tiss. Biol. 8, 213–219 (2014). https://doi.org/10.1134/S1990519X14030043

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  • DOI: https://doi.org/10.1134/S1990519X14030043

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