Abstract
It was established that UV light (240–390 nm) at doses of 151, 1510, and 3020 J/m2; reactive oxygen species (ROS); and singlet oxygen induce the DNA fragmentation of human lymphocytic cells 20 h after exposure. Using DNA comet assay, DNA damage (monostrand breaks) has been revealed to occur immediately after the UV irradiation of lymphocytes at doses of 1510 and 3020 J/m2 or after the addition of hydrogen peroxide at a concentration of 10−6 mol/l (type-C1 comets) and to reach a maximum 6 h after action on cells of UV light or ROS (type-C2 and -C3 comets). A suggestion has been made about the leading role of the p53-dependent pathway in apoptosis in human lymphocytes under the conditions of the action of UV light and ROS.
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Original Russian Text © V.G. Artyukhov, M.S. Trubitsyna, M.A. Nakvasina, E.V. Solov’eva, 2011, published in Tsitologiya, Vol. 53, No. 1, 2011, pp. 61–67.
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Artyukhov, V.G., Trubitsyna, M.S., Nakvasina, M.A. et al. DNA fragmentation of human lymphocytes in dynamics of development of apoptosis induced by action of UV radiation and reactive oxygen species. Cell Tiss. Biol. 5, 127–135 (2011). https://doi.org/10.1134/S1990519X11020039
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DOI: https://doi.org/10.1134/S1990519X11020039