Abstract
It was shown that the cytokinin content in the xylem sap of a wheat plant treated with exogenous zeatin was about ten times lower than in the nutrient solution in 24 h. Cytokinins were accumulated in roots rather shoots of treated plants. These data demonstrate the existence of a barrier in the cytokinin pathway from the nutrient solution to plant shoots. The deposition of lignin and suberin in stele detected with Sudan @III is enlarged with an increase in the distance from the tip of the root. The augmented content of suberin and lignin coincided with reduced cytokinin immunolabeling in root cells revealed by monoclonal antibodies to cytokinin and secondary gold-labeled antibodies. The accumulation of exogenous cytokinin in root stele cells shows that Casparian bands are not the only barrier on the cytokinin pathway to plant shoots. Intensive cytokinin immunolabeling in parenchyma cells surrounding stele vessels indicates the accumulation of cytokinin by these cells and suggests that there are mechanisms that limit the hormone loading in xylem vessels during transport to the shoot. The role of cytokinin transporters in this process is discussed.
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Original Russian Text © G.R. Akhiyarova, T.N. Arkhipova, 2010, published in Tsitologiya, Vol. 52, No. 12, 2010, pp. 1024–1031.
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Akhiyarova, G.R., Arkhipova, T.N. Exogenous zeatin accumulation in wheat-root cells and its role in regulation of cytokinin transport. Cell Tiss. Biol. 5, 68–73 (2011). https://doi.org/10.1134/S1990519X11010020
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DOI: https://doi.org/10.1134/S1990519X11010020