Abstract
The chemical composition of water-ethanol extracts of the brown algae Laminaria cichorioides, Costaria costata, and Fucus evanescens were studied. The extracts contained mannitol, iodine, micro elements, free amino acids, glycolipids, polyunsaturated fatty acids, fucosterine, and polyphenols. The extracts were distinguished by high contents of mannitol and iodine (L. cichorioides), lipophilic matter (C. costata), and polyphenol compounds (F. evanescens). All the extracts under study inhibited the growth of DLD-1 and HT-29 human intestine tumor cells. The strongest inhibitory effect was exerted by the extract of F. evanescens at a concentration of 50 μg/ml. The extracts can be recommended for the production of fucosterol, phlorotannins, chlorophyll derivatives, mannitol, and compositions for medical and veterinary application.
Similar content being viewed by others
References
Bagirova, V.L. and Severtsev, V.A., Nastoiki, ekstrakty, eliksiry i ikh stadartizatsiya (Tincture, Extracts, Elixirs, and Their Stadardization), SPb: SpetsLit, 2001.
Barashkov, G.K., Sravnitel’naya biokhimiya vodoroslei (Comparative Biochemistry of Seaweeds), M.: Pishchevaya prom-st’, 1972.
Vishnevskaya, T.I. and Podkorytova, A.V., Chemical Composition of Extracts Containing Iodine and Other Biologically Active Components of Brown Algae, Materialy 2 mezhdunarod. konf. “Morskie pribrezhnye ecosistemy: vodorosli, bespozvonochnye i produkti ikh pererabotki” (Materials of the 2nd International Conference “Marine Coastal Ecosystems: Seaweeds, Invertebrates, and Their Preparations”), M.: Izd-vo VNIRO, 2005, pp. 278–283.
Gusarova, I.S., Sukhoveeva, M.V., and Morgutova, I.A., Annotirovannyi spisok vodoroslei-makrofitov severnogo Primorya (Annotated List of Macrophyte Algae of the Northern Primorye), Izv. TINRO, 2000, vol. 127, pp. 626–641.
Kizevetter, I.V., Biokhimiya syr’ya vodnogo proiskhozhdeniya (Biochemitry of Raw Materials of Aquatic Origin), M.: Pishchevaya prom-st’, 1973.
Kizevetter, I.V., Gryuner, V.S., and Evtushenko, V.A., Pererabotka morskikh vodoroslei i drugikh promyslovykh vodnykh rastenii (Products from Marine Algae and Other Commercial Aquatic Plants), Moscow: Pishchevaya prom-st’, 1967.
Klochkova, N.G. and Berezovskaya, V.A., Vodorosli kamchatskogo shelfa. Rasprostranenie, biologiya, khimicheskii sostav (Seaweeds of Kamchatka Shelf. Distribution, Biology, Chemical Composition), Vladivostok; Petropavlovsk-Kamchatskii: Dalnauka, 1997.
Kovtun, I.V., The Effect of Mineral Iodine and Iodine of Food Plant Origin upon the Thyroid Gland, Problemy patologii v experimente i v klinike (Problems of Pathology in Experiment and in Clinic), Moscow: Medicine, 1974, pp. 233–239.
Orudzhev, Ya.S. and Rostovshchikov, I.I., Application of Mediatory Amino Acids (Taurin) in the Ambulatory Gerontological Praxis, Sotsialnaya i klinicheskaya psikhiatriya (Social and Clinical Psychiatry), 1998, no. 3, pp. 78–81.
Patent RF no. 2034560, MKI A 61 K 35/80. Sredstvo dlya profilaktiki raka “Klamin” (“Klamin”, “Medicine for Cancer Prophylactics”), published on May 18, 1993.
Patent RF no. 2031654, MKI A 61 K 35/80. Sredstvo dlya profilaktiki zlokachetvennikh novoobrazovanii (“Fitolon”, Medicine for Prophylactics of Malignant Neoplasms), published on May 18, 1993.
Saenko, G. N., Metally i galogeny v morskikh organizmakh (Metals and Halogens in Marine Organisms), Moscow: Nauka, 1992.
Sukhoveeva, M.V. and Podkorytova, A.V., Promyslovye vodorosli i travy morei Dal’nego Vostoka: Biologiya, rasprostranenie, zapasy, tekhnologiya pererabotki (Commercial Species of Sea Algae and Grasses of the Far-Eastern seas: Biology, Distribution, Reserves, and Technology of Treatment), Vladivostok: TINRO-Center, 2006.
Tolkunov, P.A. and Sapronov, N.S., Cardioprotective Action of Taurin, Eksperimentalnaya i klinicheskaya farmakologiya (Experimental and Clinical Pharmacology), 1997, vol. 60, no. 5, pp. 72–77.
Usov, A.I., Smirnova, G.P., and Klochkova, N.G., Polysaccharides of Algae. 55. Polysaccharide Composition of Some Brown Algae of Kamchatka, Bioorganicheskaya Khimiya, 2001, vol. 27, no. 6, pp. 444–448.
Khavezov, I. and Tsalev, D., Atomno-absorbtsionnyi analiz (Atom-Absorption Analysis), Leningrad: Khimiya, 1983.
Khotimchenko, S.V., Lipidy morskikh vodoroslei-makrofitov i trav. Struktura, raspredelenie, analiz (Lipids of Sea Macrophytic Algae and Grasses. Structure, Distribution, Analysis), Vladivostok: Dalnauka, 2003.
Alekseyenko, T.V., Zhanayev, S.Y., Venediktova, A.A. et al., Antitumor and Antimetastatic Activity of Fucoidan, a Sulfated Polysaccharide Isolated from the Okhotsk Sea Fucus evanescens Brown Algae, Bull. Exp. Biol. Med., 2007, vol. 143, pp. 730–732.
Cann, S.A., Netten, J.P. van, and Netten, C. van, Hypothesis: Iodine, Selenium and the Development of Breast Cancer, Cancer Causes Control, 2000, vol. 11, pp. 121–127.
Colburn, N.H., Wendel, E.J., and Abruzzo, G., Dissociation of Mitogenesis and Late-Stage Promotion of Tumor Cell Phenotype by Phorbol Esters: Mitogen-Resistant Variants Are Sensitive to Promotion, Proc. Natl. Acad. Sci. USA, 1981, vol. 78, no. 11, pp. 6912–6916.
Dubois, M., Gilles, K.A., Hamilton, J.K., Reiber, P.A., and Smith, F., Colorimetric Method for Determination of Sugars and Related Substances, Analyt. Chem., 1956, vol. 2, pp. 350–356.
Funahashi, H., Imai, T., Mase, T. et al., Seaweed Prevents Breast Cancer? Jpn. J. Cancer Res., 2001, vol. 92, no. 5, pp. 483–487.
Honya, M., Kinoshita, T., Ishikawa, M., Mori, H., and Nisizawa, K., Seasonal Variation in the Lipid Content of Cultured Laminaria japonica: Fatty Acids, Sterols, β-Carotene and Tocopherol, J. Appl. Phycol., 1994, vol. 6, pp. 25–29.
Hou, X., Yan, X., and Chai, C., Chemical Species of Iodine in Some Seaweeds. II. Iodinebound Biological Macromolecules, J. Radioanalyt. Nucl. Chem., 2000, vol. 245, no. 3, pp. 461–467.
Imbs, A.B., Demidkova, D.A., Latypov, Y.Y., and Pham, L.Q., Application of Fatty Acids for Chemotaxonomy of Reef-Building Corals, Lipids, 2007, vol. 42, no. 11, pp. 1035–1046.
Kotake-Nara, E., Kushiro, M., Zhang, H. et al., Carotenoids Affect Proliferation of Human Prostate Cancer Cells, J. Nutr., 2001, vol. 131, pp. 3303–3306.
Lowry, O.H., Rosebrough, N.J., Farr, A.L., and Randall, R.I., Protein Measurement with the Folin Phenol Reagent, J. Biol. Chem., 1951, vol. 193, pp. 265–275.
Matsuyama, K., Abe, K., and Kaneko, T., Seasonal Variation in Chemistry Components from Laminaria religiosa, Jpn. J. Phycol., 1982, vol. 30, no. 2, pp. 134–138.
Nakamura, T., Nagayama, K., Uchida, K., and Tanaka, R., Antioxidant Activity of Phlorotannins from the Brown Alga Eisenia bicyclis, Fish. Sci., 1996, vol. 62, no. 6, pp. 923–926.
Ohta, K., Mizushina, Y., Yamazaki, T. et al., Specific Interaction Between an Oligosaccharide on the Tumor Cell Surface and the Novel Antitumor Agents, Sulfoquinovosylacylglycerols, Biochem. Biophys. Res. Commun., 2001, vol. 288, no. 4, pp. 893–900.
Podkorytova, A.V., Chemical Composition and Exploitation of Brown Algae of the Far East Coast, Europ. Meeting: Marine Phytobenthos Studies and Their Application, Taranto, 1990, p. 85.
Ragan, M.A. and Glombitza, K.V., Phlorotannins, Brown Algal Polyphenols, Progr. Phycol. Res., Bristol: Biopress, 1986, vol. 4, pp. 129–241.
Santalova, E.A., Makarieva, T.N., Gorshkova, I.A. et al., Sterols from Six Marine Sponges, Biochem. System. Ecol., 2004, vol. 32, pp. 153–167.
Van Alstyne, K.L., A Comparison of Three Methods for Quantifying Brown Algal Polyphenolic Compounds, J. Chem. Ecol., 1995, vol. 21, pp. 45–58.
Vaskovsky, V.E. and Isay, S.V., Quantitative Determination of Formaldehyde Liberated with Periodate Oxidation, Analyt. Biochem., 1969, vol. 30, pp. 25–31.
Zvyagintseva, T.N., Shevchenko, N.M., Popivnich, I.B. et al., A New Procedure for the Separation of Water-Soluble Polysaccharides from Brown Seaweeds, Carbohydr. Res., 1999, no. 322, pp. 32–39.
Author information
Authors and Affiliations
Corresponding author
Additional information
Original Russian Text © T.I. Imbs, N.P. Krasovskaya, S.P. Ermakova, T.N. Makarieva, N.M. Shevchenko, T.N. Zvyagintseva, 2009, published in Biologiya Morya.
Rights and permissions
About this article
Cite this article
Imbs, T.I., Krasovskaya, N.P., Ermakova, S.P. et al. Comparative study of chemical composition and antitumor activity of aqueous-ethanol extracts of brown algae Laminaria cichorioides, Costaria costata, and Fucus evanescens . Russ J Mar Biol 35, 164–170 (2009). https://doi.org/10.1134/S1063074009020084
Received:
Published:
Issue Date:
DOI: https://doi.org/10.1134/S1063074009020084