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Effect of 5-azaC on the growth, flowering time and sexual phenotype of spinach

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Abstract

Spinach (Spinacia oleracea L.) is a diploid dioecious plant with a pair of homomorphic sex chromosomes X and Y. Plant DNA methylation, a known process for genome epigenetic modification, regulates gene expression in plants. To explore the effects of DNA methylation on spinach growth and sexual development, spinach seeds were treated with the demethylating reagent 5-azaC. The resulting phenotypes were then investigated, including germination percentage, root length, plant height, flowering time, and sexual phenotype. Results showed that 5-azaC at a low concentration (30 µM) only slightly influenced spinach development but promoted seed germination. The germination percentage, root length, and plant height negatively correlated with 5-azaC at 100–1000 µM. The flowering time significantly reduced at all four treatments with 5-azaC. In addition, 5-azaC influenced the sexual phenotype of spinach and remarkably increased the percentage of monoecious individuals. These results may suggest that vegetative and reproductive growth are both epigenetically regulated by DNA methylation.

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Abbreviations

5-azaC:

5-azacytidine

5mC:

cytosine methylation

MS-ISSR:

methylation-sensitive-inter-simple sequence repeats

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Authors and Affiliations

  1. College of Life Sciences, Henan Normal University, Xinxiang, 453007, China

    S. F. Li, J. H. Yuan, C. L. Deng, L. D. Lu & W. J. Gao

  2. Research Center for Immunology, Department of Laboratory Medicine, Xinxiang Medical University, Xinxiang, 453003, China

    G. J. Zhang

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  1. S. F. Li
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  2. G. J. Zhang
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  3. J. H. Yuan
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  4. C. L. Deng
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  5. L. D. Lu
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  6. W. J. Gao
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Correspondence to W. J. Gao.

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Li, S.F., Zhang, G.J., Yuan, J.H. et al. Effect of 5-azaC on the growth, flowering time and sexual phenotype of spinach. Russ J Plant Physiol 62, 670–675 (2015). https://doi.org/10.1134/S1021443715050118

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  • DOI: https://doi.org/10.1134/S1021443715050118

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