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Antioxidant responses of pea genotypes to zinc deficiency

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Abstract

The effects of Zn deficiency on antioxidant responses of two pea (Pisum sativum L.) genotypes, a Zn-efficient IPFD-99-13 and Zn-inefficient KPMR-500, grown in sand culture were studied. In the pea genotype KPMR-500, Zn deficiency decreased dry matter yield, tissue Zn concentration, and antioxidant enzyme activities istronger than in the genotype IPFD-99-13. Genotype IPFD-99-13 developed more efficient antioxidant system to scavenge ROS than genotype KPMR-500. Zinc deficiency produced oxidative damage to pea genotypes due to enhanced accumulation of TBARS and H2O2 and decreased activities of antioxidant enzymes (Cu/Zn superoxide dismutase (SOD), catalase (CAT), peroxidase (POD), and ascorbate peroxidase (APX)). In the leaves of IPFD-99-13 genotype, the higher activity of ROS-scavenging enzyme, e.g., SOD, CAT, POD, and glutathione reductase, and antioxidants, such as ascorbate and non-protein thiols, led to the lower accumulation of H2O2 and lipid peroxides. These results suggest that, by maintaining an efficient antioxidant defense system, the IPFD-99-13 genotype shows a lower sensivity to Zn deficiency than the KPMR-500 genotype.

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Abbreviations

APX:

ascorbate peroxidase

CA:

carbonic anhydrase

Car:

carotenoids

CAT:

catalase

Chl:

chlorophyll

Cu/Zn SOD:

copper/zinc superoxide dismutase

DHAR:

dehydroascorbate reductase

GR:

glutathione reductase

NPT:

non-protein thiols

POD:

peroxidase

TBARS:

thiobarbituric acid-reactive substances

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Authors and Affiliations

  1. Plant Nutrition and Stress Physiology Laboratory, Department of Botany, University of Lucknow, Lucknow, 226007, India

    N. Pandey, B. Gupta & G. C. Pathak

Authors
  1. N. Pandey
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  2. B. Gupta
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  3. G. C. Pathak
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Corresponding author

Correspondence to N. Pandey.

Additional information

Published in Fiziologiya Rastenii, 2012, Vol. 59, No. 2, pp. 225–231.

This text was submitted by the authors in English.

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Pandey, N., Gupta, B. & Pathak, G.C. Antioxidant responses of pea genotypes to zinc deficiency. Russ J Plant Physiol 59, 198–205 (2012). https://doi.org/10.1134/S1021443712010141

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