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Conformational dynamics of the tetrameric hemoglobin molecule as revealed by hydrogen exchange: I. Effects of pH, temperature, and ligand binding

  • Structural-Functional Analysis of Biopolymers and Their Complexes
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Abstract

IR spectroscopy was used to study the rate of hydrogen-deuterium (H-D) exchange of peptide NH atoms in different forms of human hemoglobin (Hb) at pH 5–10 and temperatures of 10–63°C. The pH dependence of the H-D exchange rate fits the EX2 mechanism. At 10–30°C, there are two pH-dependent conformers of liganded Hb forms, the fluctuation probability being lower for the alkaline conformer. The differences between the conformers disappear at 40°C, where a third conformer, with a higher probability of local fluctuations, appears. Deoxyhemoglobin has no pH-dependent conformers in the pH range 6–9 at 20°C, and the probability of local fluctuations is considerably decreased compared to the acid conformer of liganded Hb. The destabilization of the liganded Hb structure by decreasing the pH to 5.0 at 20°C or increasing the temperature to 50–60°C at pH 7.1 enhances global fluctuations of the native structure ensuring the H-D exchange of slowly exchanging NH atoms. The mechanisms of local and high-temperature global fluctuations, as well as the possible similarity between the two pH-dependent conformers of liganded Hb and its functional R and R2 states revealed by X-ray analysis and NMR spectroscopy, are discussed.

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Authors and Affiliations

  1. Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow, 119991, Russia

    L. V. Abaturov, N. G. Nosova & S. V. Shlyapnikov

  2. Institute of Biology, Kazan Research Center, Russian Academy of Sciences, Kazan, 420111, Tatarstan, Russia

    D. A. Faizullin

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  1. L. V. Abaturov
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  2. N. G. Nosova
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  3. S. V. Shlyapnikov
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  4. D. A. Faizullin
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Original Russian Text © L.V. Abaturov, N.G. Nosova, S.V. Shlyapnikov, D.A. Faizullin, 2006, published in Molekulyarnaya Biologiya, 2006, Vol. 40, No. 2, pp. 326–340.

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Abaturov, L.V., Nosova, N.G., Shlyapnikov, S.V. et al. Conformational dynamics of the tetrameric hemoglobin molecule as revealed by hydrogen exchange: I. Effects of pH, temperature, and ligand binding. Mol Biol 40, 284–297 (2006). https://doi.org/10.1134/S0026893306020154

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  • DOI: https://doi.org/10.1134/S0026893306020154

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