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Capsular and extracellular polysaccharides of the diazotrophic rhizobacterium Herbaspirillum seropedicae Z78

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Abstract

The diazotrophic endophyte Herbaspirillum seropedicae Z78 was shown to possess a capsule containing two high-molecular-weight glycolipids, one of which was of a lipopolysaccharide nature. These glycolipids differed considerably in the fatty acid composition of their lipid components. The polysaccharide moiety of these glycans was composed of glucose, galactose, glucosamine, galactosamine, and a noncarbohydrate component, butanetetraol. In the culture liquid of H. seropedicae Z78, an extracellular polysaccharide and an extracellular form of lipopolysaccharide were revealed. Fatty acid composition of the extracellular lipopolysaccharide differed from that of the capsular glycoconjugates; the polysaccharide moiety of exoglycans contained only neutral sugars (mannose, glucose, and galactose) and a tetraatomic alcohol, butanetetraol. It is assumed that structural diversity of polysaccharide-containing polymers at the surface of H. seropedicae Z78 cells is conditioned by their different roles in plant colonization and formation of efficient symbiosis.

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Authors and Affiliations

  1. Institute of Biochemistry and Physiology of Plants and Microorganisms, Russian Academy of Sciences, pr. Entuziastov 13, Saratov, 410049, Russia

    O. N. Smol’kina & N. S. Shishonkova

  2. Chernyshevsky Saratov State University, ul. Astrakhanskaya 83, Saratov, 410012, Russia

    N. A. Yurasov & V. V. Ignatov

Authors
  1. O. N. Smol’kina
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  2. N. S. Shishonkova
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  3. N. A. Yurasov
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  4. V. V. Ignatov
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Correspondence to O. N. Smol’kina.

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Original Russian Text © O.N. Smol’kina, N.S. Shishonkova, N.A. Yurasov, V.V. Ignatov, 2012, published in Mikrobiologiya, 2012, Vol. 81, No. 3, pp. 345–352.

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Smol’kina, O.N., Shishonkova, N.S., Yurasov, N.A. et al. Capsular and extracellular polysaccharides of the diazotrophic rhizobacterium Herbaspirillum seropedicae Z78. Microbiology 81, 317–323 (2012). https://doi.org/10.1134/S0026261712030113

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  • DOI: https://doi.org/10.1134/S0026261712030113

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