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Capsular polysaccharide of the bacterium Azospirillum lipoferum Sp59b: Structure and antigenic specificity

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Abstract

Antigenic differences were revealed between the cell wall outer membrane lipopolysaccharides and the capsular high molecular weight bioglycans for a typical strain of the nitrogen-fixing rhizobacterium Azospirillum lipoferum Sp59b using antibodies prepared against the homologous lipopolysaccharide and lipopolysaccharide-protein complex. From the capsular lipopolysaccharide-protein and polysaccharide-lipid complexes of A. lipoferum Sp59b, polysaccharides were isolated and their structure was for the first time established in Azospirillum by monosaccharide analysis which included determination of the absolute configurations, methylation, O-deacetylation, and one- and two-dimensional NMR spectroscopy. The polysaccharides of the capsular complexes were shown to have identical structure of the branched tetrasaccharide repeating unit, which differs from the structure of the O-specific polysaccharide within the outer membrane lipopolysaccharide of this strain.

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Abbreviations

Ab:

antibodies

COSY:

correlation spectroscopy

CPS:

capsular polysaccharides

DPS:

O-deacetylated polysaccharide

EIA:

enzyme immunoassay

HMBC:

heteronuclear multiple bond correlation

HSQC:

heteronuclear single quantum coherence

KDO:

2-keto-3-deoxyoctonic acid

LPPC:

lipopolysaccharide-protein complex

LPS:

lipopolysaccharide (O-antigen)

OPS:

O-specific polysaccharide

PS:

polysaccharide

PSLC:

polysaccharide-lipid complex

ROESY:

rotation nuclear Overhauser effect spectroscopy

TOCSY:

total correlation spectroscopy

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Authors and Affiliations

  1. Institute of Biochemistry and Physiology of Plants and Microorganisms, Russian Academy of Sciences, pr. Entuziastov 13, 410049, Saratov, Russia

    O. N. Smol’kina, Yu. P. Fedonenko, G. L. Burygin, L. Yu. Matora, S. A. Konnova & V. V. Ignatov

  2. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Leninsky pr. 47, 119991, Moscow, Russia

    V. V. Kachala & E. L. Zdorovenko

  3. Saratov State University, ul. Astrakhanskaya 83, 410012, Saratov, Russia

    S. A. Konnova

Authors
  1. O. N. Smol’kina
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  2. V. V. Kachala
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  3. Yu. P. Fedonenko
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  4. G. L. Burygin
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  5. E. L. Zdorovenko
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  6. L. Yu. Matora
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  7. S. A. Konnova
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  8. V. V. Ignatov
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Correspondence to O. N. Smol’kina.

Additional information

Original Russian Text © O. N. Smol’kina, V. V. Kachala, Yu. P. Fedonenko, G. L. Burygin, E. L. Zdorovenko, L. Yu. Matora, S. A. Konnova, V. V. Ignatov, 2010, published in Biokhimiya, 2010, Vol. 75, No. 5, pp. 707–716.

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Smol’kina, O.N., Kachala, V.V., Fedonenko, Y.P. et al. Capsular polysaccharide of the bacterium Azospirillum lipoferum Sp59b: Structure and antigenic specificity. Biochemistry Moscow 75, 606–613 (2010). https://doi.org/10.1134/S000629791005010X

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  • DOI: https://doi.org/10.1134/S000629791005010X

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