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Methods for selection of aptamers to protein targets

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Abstract

Aptamers are synthetic single-stranded RNA or DNA molecules capable of specific binding to other target molecules. In this review, the main aptamer properties are considered and methods for selection of aptamers against various protein targets are described. Special attention is given to the methods for directed selection of aptamers, which allow one to obtain ligands with specified properties.

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Abbreviations

NES:

nuclear export signal

nt:

nucleotide

PCR:

polymerase chain reaction

SELEX:

systematic evolution of ligands by exponential enrichment

ssDNA:

single-stranded DNA molecule

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  1. Institute of Molecular Genetics, Russian Academy of Sciences, pl. Kurchatova 2, 123182, Moscow, Russia

    A. V. Kulbachinskiy

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Original Russian Text © A. V. Kulbachinskiy, 2006, published in Uspekhi Biologicheskoi Khimii, 2006, Vol. 46, pp. 193–224.

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Kulbachinskiy, A.V. Methods for selection of aptamers to protein targets. Biochemistry Moscow 72, 1505–1518 (2007). https://doi.org/10.1134/S000629790713007X

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