Abstract
The ability of the Alcaligenes faecalis 2 strain to utilize acrylamide and acrylic acid upon cultivation with these compounds as the only sources of carbon and energy has been investigated. Complete utilization of the acrylic acid present in the medium at concentrations below 0.113 g/L was observed by cultivation day 5, at a concentration of 0.225 g/L by day 7, and at a concentration of 0.45 g/L by day 17. Complete utilization of the acrylamide present in the medium at concentrations below 0.4 g/L was observed by day 5, at a concentration of 0.9 g/L by day 7, and at a concentration of 1.8 g/L by day 20. Importantly, bacterial growth did not start before complete transformation of acrylamide into acrylic acid. The rate of acrylamide transformation by growing bacteria and a cell suspension in the stationary growth phase amounted to 12.5 mg/L h at a cell concentration of 610 mg/L and 300 mg/L h, at a concentration of 1500 mg/L. A. faecalis 2 cells immobilized on BVV-22 basalt fibers and Carbopon-B-aktiv at concentrations of 3000 and 800 mg dry cells/L, respectively, transformed acrylamide at a rate of 1200 mg/L h.
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Beerthuis, R., Rothenberg, G., and Shiju, N.R., Green Chem, 2015, vol. 17, no. 3, pp. 1341–1361.
Ahmad, M.A.A., Kamaruzzaman, M.R., and Chin, S.Y., Process. Saf. Environ. Prot., 2014, vol. 92, no. 6, pp. 522–531.
Kusnin, N., Syed, M.A., and Ahmad, S.A., JOBIMB, 2015, vol. 3, no. 2, pp. 6–12.
Charoenpanich, J., Removal of acrylamide by microorganisms, in Applied Bioremediation—Active and Passive Approaches, Patil, Y.B. and Rao, P., Eds., InTech Open Science, 2013, pp. 101–121. doi 10.5772/56150
Rivas-Jimenez, L., Ramírez-Ortiz, K., González-Córdova, A.F., Vallejo-Cordoba, B., Garcia, H.S., and Hernandez-Mendoza, A., Microbiol. Res., 2016, vol. 190, pp. 19–26.
Shamla, L. and Nisha, P., Food Chem., 2017, vol. 222, pp. 53–60.
Nguyen, H.T., van der Fels-Klerx, H.J., (I.), and van Boekel, M.A.J.S., Food Chem., 2017, vol. 230, pp. 14–23.
Wu, X., Liu, C.-Y., and Li, P.-F., Mar. Chem., 2015, vol. 170, pp. 29–36.
Chu, H.S., Ahn, J.-H., Yun, J., Choi, I.S., Nam, T.-W., and Cho, K.M., Metab. Eng., 2015, vol. 32, pp. 23–29.
Debabov, V.G. and Yanenko, A.S., Obzor. Zh. Khim., 2011, vol. 1, no. 4, pp. 376–394.
Buranasilp, K. and Charoenpanich, J., J. Environ. Sci., 2011, vol. 23, no. 3, pp. 396–403.
Jebasingh, S.E.J., Lakshmikandan, M., Rajesh, R.P., and Raja, P., Int. Biodeterior. Biodegrad., 2013, vol. 85, pp. 120–125.
Lakshmikandan, M., Sivaraman, K., Raja, S.E., Vasanthakumar, P., Rajesh, R.P., Sowparthani, K., and Jebasingh, S.E.J., Int. Biodeterior. Biodegrad., 2014, vol. 94, pp. 214–221.
Shen, S.-M., Wan, T.-J., and Hwang, H.-Y., Biocatal. Agric. Biotechnol., 2012, vol. 1, no. 2, pp. 110–114.
Wampler, D.A. and Ensign, S.A., Appl. Environ. Microbiol., 2005, vol. 71, no. 10, pp. 5850–5857.
Wang, C.C. and Lee, C.M., Water Sci. Technol., 2006, vol. 53, no. 6, pp. 181–186.
Wang, C.C., Lee, C.M., and Wu, A.S., Water Sci. Technol., 2009, vol. 60, no. 11, pp. 311–316.
Wang, C.-C., Lee, C.-M., and Wu, A.-S., Sustain. Environ. Res., 2014, vol. 24, no. 4, pp. 311–315.
Demakov, V.A., Vasil’ev, D.M., Maksimova, Yu.G., Pavlova, Yu.A., Ovechkina, G.V., and Maksimov, A.Yu., Microbiology (Moscow), 2015, vol. 84, no. 3, pp. 433–441.
Maksimova, Yu.G., Gorbunova, A.N., Zorina, A.S., Maksimov, A.Yu., Ovechkina, G.V., and Demakov, V.A., Appl. Biochem. Microbiol., 2015, vol. 51, no. 1, pp. 64–69.
Maier, R.M., Environmental Microbiology, 2nd ed., Pepper, I.L. Gerba, C.P., Gentry, T., and Maier, R.M., Eds., Elsevier Inc., 2009, pp. 37–54.
Liu, Y. and Wang, Z.-W., Aerobic granulation in sequencing batch reactors, in Wastewater Purification, Liu, Y., Ed., Boca Raton, FL: CRC Press, 2008, pp. 149–181.
Fournand, D. and Arnaud, A., J. Appl. Microbiol., 2001, vol. 91, no. 3, pp. 381–393.
Zödl, B., Schmid, D., Wassler, G., Gundacker, C., Leibetseder, V., Thalhammer, T., and Ekmekcioglu, C., Toxicology, 2007, vol. 232, nos. 1–2, pp. 99–108.
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Original Russian Text © Yu.G. Maksimova, D.M. Vasil’ev, A.S. Zorina, G.V. Ovechkina, A.Yu. Maksimov, 2018, published in Prikladnaya Biokhimiya i Mikrobiologiya, 2018, Vol. 54, No. 2, pp. 158–164.
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Maksimova, Y.G., Vasil’ev, D.M., Zorina, A.S. et al. Acrylamide and Acrylic Acid Biodegradation by Alcaligenes faecalis 2 Planktonic Cells and Biofilms. Appl Biochem Microbiol 54, 173–178 (2018). https://doi.org/10.1134/S0003683818020084
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DOI: https://doi.org/10.1134/S0003683818020084