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Regulation of the binding of the BMP-2 growth factor with collagen by blood plasma fibronectin

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Abstract

The release kinetics of recombinant human bone morphogenic factor 2 (rhBMP-2) from collagen hydrogel in the presence of human blood plasma have been studied. The expulsion of rhBMP-2 from the collagen-BMP-2 complex by the competitive adhesion of collagen-binding proteins penetrating from plasma was firstly recognized. It was experimentally proven that that blood plasma fibronectin is the main collagen-binding protein, which is responsible for the controlled release of rhBMP-2. As a result, a new collagen hydrogel with the incorporation of fibronectin was created which retained rhBMP-2 for a twice longer period as compared to the ordinary collagen hydrogel. A distinctive feature of this new collagen-fibronectin matrix is the slow release of rhBMP-2 in the first three days which allows for the avoiding of adverse effects in clinics caused by the rapid release of large amounts of rhBMP-2 from collagen hydrogel.

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Correspondence to E. O. Osidak.

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Original Russian Text © E.O. Osidak, M.S. Osidak, D.E. Sivogrivov, T.S. Portnaya, T.M. Grunina, L.A. Soboleva, V.G. Lunin, A.S. Karyagina, S.P. Domogatskii, 2014, published in Prikladnaya Biokhimiya i Mikrobiologiya, 2014, Vol. 50, No. 2, pp. 226–231.

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Osidak, E.O., Osidak, M.S., Sivogrivov, D.E. et al. Regulation of the binding of the BMP-2 growth factor with collagen by blood plasma fibronectin. Appl Biochem Microbiol 50, 200–205 (2014). https://doi.org/10.1134/S0003683814020148

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