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Inducible specific lux-biosensors for the detection of antibiotics: Construction and main parameters

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Abstract

Based on Escherichia coli, highly sensitive specific lux-biosensors for the detection of tetracycline and β-lactam antibiotics, quinolones, and aminoglycosides have been obtained. To make biosensors, bacteria were used that contained hybrid plasmids pTetA’::lux, pAmpC’::lux, pColD’::lux, and pIbpA’::lux, in which transcription of the reporter Photorhabdus luminescens luxCDABE genes occurred from the inducible promoters of the tetA, ampC, cda, and ibpA genes, respectively. The main parameters (threshold sensitivity and response time) of lux-biosensors were measured. The high specificity of biosensors responding only to antibiotics of a certain type was demonstrated.

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Authors and Affiliations

  1. State Research Institute for Genetics and Selection of Industrial Microorganisms, Moscow, 117545, Russia

    V. Yu. Kotova, K. V. Ryzhenkova, I. V. Manukhov & G. B. Zavilgelsky

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  1. V. Yu. Kotova
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  2. K. V. Ryzhenkova
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  3. I. V. Manukhov
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  4. G. B. Zavilgelsky
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Corresponding author

Correspondence to G. B. Zavilgelsky.

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Original Russian Text © V.Yu. Kotova, K.V. Ryzhenkova, I.V. Manukhov, G.B. Zavilgelsky, 2014, published in Prikladnaya Biokhimiya i Mikrobiologiya, 2014, Vol. 50, No. 1, pp. 112–117.

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Kotova, V.Y., Ryzhenkova, K.V., Manukhov, I.V. et al. Inducible specific lux-biosensors for the detection of antibiotics: Construction and main parameters. Appl Biochem Microbiol 50, 98–103 (2014). https://doi.org/10.1134/S0003683814010074

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