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Screening of taxol-producing endophytic fungi from Taxus chinensis var. mairei

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Abstract

A total of 38 endophytic fungus strains were isolated from Taxus chinensis var. mairei by the aseptic technique. Genomic DNA was extracted from isolated endophytic fungi and subjected to polymerase chain reaction (PCR) analysis for the presence of the Taxus taxadiene synthase (TS) gene, a rate-limiting enzyme gene in the taxol biosynthetic pathway. Twelve out of 38 isolated endophytic fungus strains showed PCR positive for the ts gene. Subsequently, taxol and its related compounds were extracted from culture filtrates and mycelia of the PCR positive strains, separated by column chromatography, and analyzed by High Performance Liquid Chromatography and Mass Spectrum. The analysis result showed that 3 strains could produce taxol and its related compounds at the detectible level. This study indicates that molecular detection of the ts gene is an efficient method for primary screening of taxol or its related compound-producing endophytic fungi, which can improve prominently screening efficiency.

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Authors and Affiliations

  1. Plant Biotechnology Research Center, School of Agriculture and Biology, Fudan-SJTU-Nottingham Plant Biotechnology R&D Center, Shanghai Jiao Tong University, Shanghai, 200030, China

    X. Zhou & K. Tang

  2. State Key Laboratory of Genetic Engineering, School of Life Sciences, Fudan-SJTU-Nottingham Plant Biotechnology R&D Center, Fudan University, Shanghai, 200433, China

    Z. Wang, K. Jiang, Y. Wei, J. Lin, X. Sun & K. Tang

Authors
  1. X. Zhou
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  2. Z. Wang
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  3. K. Jiang
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  4. Y. Wei
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  5. J. Lin
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  6. X. Sun
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  7. K. Tang
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Published in Russian in Prikladnaya Biokhimiya i Mikrobiologiya, 2007, Vol. 43, No. 4, pp. 490–494.

The text was submitted by the authors in English.

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Zhou, X., Wang, Z., Jiang, K. et al. Screening of taxol-producing endophytic fungi from Taxus chinensis var. mairei . Appl Biochem Microbiol 43, 439–443 (2007). https://doi.org/10.1134/S000368380704014X

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  • DOI: https://doi.org/10.1134/S000368380704014X

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