Label-free detection of biomolecules using a tapered optical fiber sensor

Ye Tian; Wenhui Wang; Armand Chery Jr.; Nan Wu; Charles Guthy; Xingwei Wang

doi:10.1117/12.852215

26 April 2010 Label-free detection of biomolecules using a tapered optical fiber sensor

Ye Tian, Wenhui Wang, Armand Chery Jr., Nan Wu, Charles Guthy, Xingwei Wang

Proceedings Volume 7673, Advanced Environmental, Chemical, and Biological Sensing Technologies VII; 767307 (2010) https://doi.org/10.1117/12.852215
Event: SPIE Defense, Security, and Sensing, 2010, Orlando, Florida, United States

Abstract

A tapered optical fiber sensor (TOFS) is a kind of optical fiber sensor that uses special geometries to measure properties of surrounding environments or samples using evanescent waves. This paper presents a fast, highly sensitive, and inexpensive tapered optical fiber biosensor that, using a miniature sensing structure, enables the label-free direct detection of biomolecules. The sensor takes advantage of the interference effect between the fiber's first two modes along the taper waist region. This effect causes some interference fringes in the transmission spectrum. Because of its sharp spectrum fringe signals and its long biomolecule testing region, the sensor is fast and highly sensitive. To better understand the influence of various biomolecules on the sensor, a computer simulation that varied such bio-layer parameters as thickness and refractive index was performed. The result of 0.4 nm/nm showed that the spectrum fringe shift of the sensor was large enough to be easily measured even when the bio-layer was nanometers thick. A tapered optical fiber biosensor was then fabricated and evaluated with an immune globulin G (IgG) antibody-antigen pair, and showed good performance.

Citation Download Citation

Ye Tian, Wenhui Wang, Armand Chery Jr., Nan Wu, Charles Guthy, and Xingwei Wang "Label-free detection of biomolecules using a tapered optical fiber sensor", Proc. SPIE 7673, Advanced Environmental, Chemical, and Biological Sensing Technologies VII, 767307 (26 April 2010); https://doi.org/10.1117/12.852215

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