Bioadhesive nanoareas in antifouling matrix for highly efficient affinity sensors

I. Mannelli; A. Valsesia; P. Lisboa; M. Anger-Leroy; S. Mornet; P. Colpo; F. Rossi

doi:10.1117/12.796969

29 August 2008 Bioadhesive nanoareas in antifouling matrix for highly efficient affinity sensors

I. Mannelli, A. Valsesia, P. Lisboa, M. Anger-Leroy, S. Mornet, P. Colpo, F. Rossi

Proceedings Volume 7035, Biosensing; 70350Y (2008) https://doi.org/10.1117/12.796969
Event: NanoScience + Engineering, 2008, San Diego, California, United States

Abstract

A procedure for fabricating nanopatterned surfaces at the sub-500 nm scale comprising a hexagonal close packed array of bioadhesive gold nanoareas in a protein resistant matrix (PEO-like polymer), has been optimized. The surfaces were characterized by AFM analysis and their interaction with amino functionalised gold nanoparticles as models were investigated. The AFM images show the crystalline arrangement of nanopattern array and the localized adsorption of the H2N-Au nanoparticles in the bioadhesive nanoareas. A Surface Plasmon Resonance imaging (SPRi) system was used to assess the detection performances of these surfaces when employed as a transduction platform for studying biomolecule interactions. The investigated surfaces showed an enhancement of the affinity reaction efficiency with respect to the non structured surfaces. The obtained preliminary results show that nanostructuring the surfaces improve the binding site accessibility of the immobilized biological probes without significantly modifying the native biomolecule conformation.

Citation Download Citation

I. Mannelli, A. Valsesia, P. Lisboa, M. Anger-Leroy, S. Mornet, P. Colpo, and F. Rossi "Bioadhesive nanoareas in antifouling matrix for highly efficient affinity sensors", Proc. SPIE 7035, Biosensing, 70350Y (29 August 2008); https://doi.org/10.1117/12.796969

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