A portable array biosensor for food safety

Joel P. Golden; Miriam M. Ngundi; Lisa C. Shriver-Lake; Chris R. Taitt; Frances S. Ligler

doi:10.1117/12.570285

19 November 2004 A portable array biosensor for food safety

Joel P. Golden, Miriam M. Ngundi, Lisa C. Shriver-Lake, Chris R. Taitt, Frances S. Ligler

Proceedings Volume 5587, Nondestructive Sensing for Food Safety, Quality, and Natural Resources; (2004) https://doi.org/10.1117/12.570285
Event: Optics East, 2004, Philadelphia, Pennsylvania, United States

Abstract

An array biosensor developed for simultaneous analysis of multiple samples has been utilized to develop assays for toxins and pathogens in a variety of foods. The biochemical component of the multi-analyte biosensor consists of a patterned array of biological recognition elements immobilized on the surface of a planar waveguide. A fluorescence assay is performed on the patterned surface, yielding an array of fluorescent spots, the locations of which are used to identify what analyte is present. Signal transduction is accomplished by means of a diode laser for fluorescence excitation, optical filters and a CCD camera for image capture. A laptop computer controls the miniaturized fluidics system and image capture. Results for four mycotoxin competition assays in buffer and food samples are presented.

Citation Download Citation

Joel P. Golden, Miriam M. Ngundi, Lisa C. Shriver-Lake, Chris R. Taitt, and Frances S. Ligler "A portable array biosensor for food safety", Proc. SPIE 5587, Nondestructive Sensing for Food Safety, Quality, and Natural Resources, (19 November 2004); https://doi.org/10.1117/12.570285

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