Reversible integrated optic evanescent field biosensor using chemical amplification for added sensitivity

Daniel P. Campbell; Nile F. Hartman; Jeffrey L. Moore; James V. Suggs; Janet M. Cobb

doi:10.1117/12.308033

1 May 1998 Reversible integrated optic evanescent field biosensor using chemical amplification for added sensitivity

Daniel P. Campbell, Nile F. Hartman, Jeffrey L. Moore, James V. Suggs, Janet M. Cobb

Proceedings Volume 3253, Biomedical Sensing and Imaging Technologies; (1998) https://doi.org/10.1117/12.308033
Event: BiOS '98 International Biomedical Optics Symposium, 1998, San Jose, CA, United States

Abstract

Planar waveguide interferometers provide an attractive sensing platform for biosensor applications. Advantages include small size, real-time sensing, multiple analyte detection on a chip, performance independent of wavelength and optical power, and nulling of thermal and mechanical noise. Limitations include slow diffusion time of the analyte to the functionalized surface, interference from non-specific binding and bulk index of refraction changes and a lack of reversibility. Combining certain techniques used in affinity chromatography and enzyme-linked immunosorbent assays and with an amplifying chemoselective film on the waveguide produces a sensor that is versatile, reusable and overcomes most of the above limitations. Work will be presented using an optical pH and ammonia sensor for detection.

Citation Download Citation

Daniel P. Campbell, Nile F. Hartman, Jeffrey L. Moore, James V. Suggs, and Janet M. Cobb "Reversible integrated optic evanescent field biosensor using chemical amplification for added sensitivity", Proc. SPIE 3253, Biomedical Sensing and Imaging Technologies, (1 May 1998); https://doi.org/10.1117/12.308033

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