Micropatterned surfaces for understanding and influencing interactions of biological systems with optical devices

Gabriel P. Lopez; Leonard M. Tender; Gail Bradley; Kimberly A. Opperman; Philip D. Hampton

doi:10.1117/12.269954

31 March 1997 Micropatterned surfaces for understanding and influencing interactions of biological systems with optical devices

Gabriel P. Lopez, Leonard M. Tender, Gail Bradley, Kimberly A. Opperman, Philip D. Hampton

Author Affiliations +

Proceedings Volume 2978, Micro- and Nanofabricated Electro-Optical Mechanical Systems for Biomedical and Environmental Applications; (1997) https://doi.org/10.1117/12.269954
Event: BiOS '97, Part of Photonics West, 1997, San Jose, CA, United States

Abstract

This paper reviews methods for forming micropatterned surfaces of self-assembled monolayers of alkylthiolates on gold and their use in studying and manipulating interactions of biological systems with solid surfaces. Optics-based biosensor arrays are an example of a technology where it is desirable to prepare microscopic patterns of well-defined surfaces that contain regions with specific bioactivity or that resist nonspecific biointeraction (e.g., nonspecific protein adsorption and cellular attachment). Several simple patterning techniques are described including photopatterning, monolayer displacement, microinjection, and electrochemical patterning. The relative merits of these techniques are discussed with regard to maintenance of bioactivity of immobilized chemical species, resistance to nonspecific bioactivity, and application to rapid, large- scale patterning. The use of electrochemical patterning is also described in the creation of a model microsensor array.

Citation Download Citation

Gabriel P. Lopez, Leonard M. Tender, Gail Bradley, Kimberly A. Opperman, and Philip D. Hampton "Micropatterned surfaces for understanding and influencing interactions of biological systems with optical devices", Proc. SPIE 2978, Micro- and Nanofabricated Electro-Optical Mechanical Systems for Biomedical and Environmental Applications, (31 March 1997); https://doi.org/10.1117/12.269954

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available