Micro-patterned quantum dots excitation for cellular microarray imaging

Gauri Bhave; Elaine Ng; Youngkyu Lee; John X.J. Zhang

doi:10.1117/12.2083645

11 March 2015 Micro-patterned quantum dots excitation for cellular microarray imaging

Gauri Bhave, Elaine Ng, Youngkyu Lee, John X.J. Zhang

Proceedings Volume 9341, Bioinspired, Biointegrated, Bioengineered Photonic Devices III; 93410F (2015) https://doi.org/10.1117/12.2083645
Event: SPIE BiOS, 2015, San Francisco, California, United States

Abstract

We present a compact light source designed for arrayed lab-on-chip cell imaging with the motivation of creating a microchip based system for detection of tumor cells. We aim at creating a multicolor light source that can be integrated for on-chip imaging. Colloidal quantum dots (QDs) were used as the emission layer due to their unique capabilities like multicolor emission, multiple available methods of electrical and photo excitation and compatibility with silicon fabrication were achieved. Micropatterning of QDs was used to create both electrically and photo excited light sources. We study the photo activated source as a robust, high intensity light source which can be easily integrated with lab-onchip systems while requiring additional filters and excitation systems and compare it with an electrically excited source with the capability of individually addressable, multicolor sources on a single substrate eliminating the need for additional optical components. To demonstrate the efficacy of our design, we performed ex vivo transmission mode microscopy to evaluate the nucleus-cytoplasm ratios of cancer cells. We showed the capability of imaging of inner cell structures using multiple wavelengths to perform high contrast imaging and observation. We performed immunofluorescence excitation of MDA-MB 231 cancer cells, cultured in a microwell array. Our method provides patterned multicolor light sources and low cost which are suitable for high-throughput microarray cellular imaging.

Citation Download Citation

Gauri Bhave, Elaine Ng, Youngkyu Lee, and John X.J. Zhang "Micro-patterned quantum dots excitation for cellular microarray imaging", Proc. SPIE 9341, Bioinspired, Biointegrated, Bioengineered Photonic Devices III, 93410F (11 March 2015); https://doi.org/10.1117/12.2083645

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available