Toxin detection using a fiber-optic-based biosensor

Robert A. Ogert; Lisa C. Shriver-Lake; Frances S. Ligler

doi:10.1117/12.144706

18 May 1993 Toxin detection using a fiber-optic-based biosensor

Robert A. Ogert, Lisa C. Shriver-Lake, Frances S. Ligler

Proceedings Volume 1885, Advances in Fluorescence Sensing Technology; (1993) https://doi.org/10.1117/12.144706
Event: OE/LASE'93: Optics, Electro-Optics, and Laser Applications in Scienceand Engineering, 1993, Los Angeles, CA, United States

Abstract

Using an evanescent wave fiber optic-based biosensor developed at Naval Research Laboratory, ricin toxin can be detected in the low ng/ml range. Sensitivity was established at 1 - 5 ng/ml using a two-step assay. The two-step assay showed enhanced signal levels in comparison to a one-step assay. A two-step assay utilizes a 10 minute incubation of an immobilized affinity purified anti-ricin antibody fiber optic probe in the ricin sample before placement in a solution of fluorophore-labeled goat anti-ricin antibodies. The specific fluorescent signal is obtained by the binding of the fluorophore-labeled antibodies to ricin which is bound by the immobilized antibodies on the fiber optic probe. The toxin can be detected directly from urine and river water using this fiber optic assay.

Citation Download Citation

Robert A. Ogert, Lisa C. Shriver-Lake, and Frances S. Ligler "Toxin detection using a fiber-optic-based biosensor", Proc. SPIE 1885, Advances in Fluorescence Sensing Technology, (18 May 1993); https://doi.org/10.1117/12.144706

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