Abstract
The microbial diversity of the intestinal tract content of the river trout from two Lithuanian rivers has been investigated by molecular methods: polymerase chain reaction amplification and sequencing of partial 16S rRNA genes. Predominant bacterial populations detected in the river trout intestinal tract from the Skorblus River were Rahnella (21%), from the Žeimena River, Aeromonas (41.7%) and Plesiomonas (22.9%). Buttiauxella agrestis, Budvicia aquatica, Erwinia persicinus, Yersinia mollaretii, Y. kristensenii, Y. rohdei, Moellerella wisconsensis, Obesumbacterium proteus, Pantoea cedenensis, Rahnella aquatillis, and Rahnella sp. from the Enterobacteriaceae family have been detected in the intestinal tract of freshwater salmonid fish for the first time.
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References
Clements KD. Fermentation and gastrointestinal micro-organisms in fishes. In: Mackie RI, White BA (eds). Gastrointestinal Microbiology, Vol. 1. Gastrointestinal Ecosystems and Fermentations. Chapman & Hall, New York. 1997; 156–198.
Ringø E, Gatesoupe FJ. Lactic acid bacteria in fish: a review. Aquaculture 1998; 160: 177–203.
Ringø E, Lødemel JB, Myklebust R, Kaino T, Mayhew TM, Olsen RE. Epithelium-associated bacteria in the gastrointestinal tract of Arctic charr (Salvelinus alpinus L.). An electron microscopical study. J. Appl. Microbiol. 2001; 90: 294–300.
Ringø E, Lødemel JB, Myklebust R, Jensen L, Lund V, Mayhew TM, Olsen RE. The effects of soybean. linseed and marine oils on aerobic gut microbiota of Arctic charr Salvelinus alpinus L. before and after challenge with Aeromonas salmonicida ssp. salmonicida. Aquacult. Res. 2002; 33: 591–606.
Ringø E, Olsen RE, Mayhew TM, Myklebust R. Electron microscopy of the intestinal microflora of fish. Aquaculture 2003; 227: 395–415.
Austin B, Al-Zahrani AMJ. The effect of antimicrobial compounds on the gastrointestinal microflora of rainbow trout, Salmo gairdneri Richardson. J. Fish Biol. 1988; 33: 1–14.
Cahill MM. Bacterial flora of fishes: a review. Microb. Ecol. 1990; 19: 21–41.
Nieto TP, Toranzo AE, Barja JL. Comparison between the bacterial flora associated with fingerlings rainbow trout cultured in two different hatcheries in the north-west of Spain. Aquaculture 1984; 42: 193–206.
Ringø E. Does dietary linoleic acid affect intestinal microflora in Arctic charr. Salvelinus alpinus (L.)? Aquacult. Fish. Manage. 1993; 24: 133–135.
Ringø E. Does chromic oxide (Cr2O3) affect faecal lipid and intestinal bacterial flora in Arctic charr Salvelinus alpinus (L)? Aquacult. Fish. Manage. 1993; 24: 767–776.
Ringø E, Strøm E. Microflora of Arctic charr. Salvelinus alpinus (L.). I. The gastrointestinal microflora of free-living fish, and the effect of diet and salinity on intestinal microflora. Aquacult. Fish. Manage. 1994; 25: 623–629.
Ringø E, Strøm E, Tabachek JA. Intestinal microflora of salmonids: a review. Aquacult. Res. 1995; 26: 773–789.
Trust TJ, Sparrow RAH. The bacterial flora in the alimentary tract of freshwater salmonid fishes. Can. J. Microbiol. 1974; 20: 1219–1228.
Trust TJ. Facultative anaerobic bacteria in the digestive tract of chum salmon (Oncorhynchus keta) maintained in fresh water under defined culture conditions. Appl. Microbiol. 1975; 29: 663–668.
Yoshimizu M, Kimura T, Sakai M. Studies on the intestinal microflora of salmonids: I. The intestinal microflora of fish reared in freshwater and seawater. Nippon Suisan Gakkaishi 1976; 42: 91–99.
Yoshimizu M, Kimura T, Sakai M. Studies on the intestinal microflora of salmonids-II. Effects of artificial transplanting from freshwater into sea water on the intestinal microflora of feeding and non-feeding fish. Nippon Suisan Gakkaishi 1976; 42: 863–873.
Yoshimizu M, Kimiyama K, Kimura T, Sakai M. Studies on the intestinal microflora of salmonids: IV. The intestinal microflora of fresh water salmon. Nippon Suisan Gakkaishi 1976; 42: 1281–1290.
Yoshimizu M, Kimura T, Sakai M. Studies on the intestinal microflora of salmonids: V. The intestinal microflora of the anadromous salmon. Nippon Suisan Gakkaishi 1976; 42: 1291–1298.
Hongoh Y, Yuzawa H, Ohkuma M, Kudo T. Evaluation of primers and PCR conditions for the analysis of 16S rRNA genes from natural environment. FEMS Microb. Lett. 2003; 221: 299–304.
Kilkus K. Geography of Lithuanian Waters. Apyaušris. Vilnius. 1998 (in Lithuanian).
Maidak BL, Cole JR, Lilburn TG, Parker CTJ, Saxman RP, Farris JR, Garrity GM, Olsen GJ, Schmidt TM, Tiedje JM. The RDP-II (Ribosomal Database Project). Nucleic Acids Res. 2001; 29: 173–174.
Stoesser G, Baker van den W, Broek A, Camon E, Garcia-Pastor M, Kanz C, Kulikova T, Lombard V, Lopez R, Parkinson H, Redasci N, Sterk P, Stoehr P, Tuli MA. The EMBL Nucleotide sequence database. Nucleic Acids Res. 2001; 29: 17–21.
Saitou N, Nei M. The neighbor joining method: a new method for constructing phylogenetic trees. Mol. Biol. Evol. 1987; 4: 406–425.
Holben WE, Williams P, Saarinen M, Särkilahti LK, Apajalahti JHA. Phylogenetic analysis of intestinal microflora indicates a novel Mycoplasma phylotype in farmed and wild salmon. Microb. Ecol. 2002; 44: 175–185.
Spanggaard B, Huber I, Nielsen J, Nielsen T, Appel KF, Gram L. The microflora of rainbow trout intestine: a comparison of traditional and molecular identification. Aquaculture 2000; 182: 1–15.
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Skrodenyte-Arbaciauskiene, V., Sruoga, A. & Butkauskas, D. Assessment of microbial diversity in the river trout Salmo trutta fario L. intestinal tract identified by partial 16S rRNA gene sequence analysis. Fish Sci 72, 597–602 (2006). https://doi.org/10.1111/j.1444-2906.2006.01189.x
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DOI: https://doi.org/10.1111/j.1444-2906.2006.01189.x