Crystallization and preliminary X-ray analysis of endopolygalacturonase SE1 from Trichosporon penicillatum

Endopolygalacturonase SE1 from Trichosporon penicillatum has been crystallized by the hanging-drop method of vapour diffusion using ammonium sulfate as a precipitant. The crystals belong to the hexagonal space group P6₁ or P6₅, with unit-cell parameters a = b = 135.0, c = 70.7 Å, γ = 120°. The calculated V_M based on one molecule per asymmetric unit is 3.09 ų Da⁻¹. A native data set from a crystal has been collected to 2.0 Å resolution on a Cu Kα rotating-anode X-ray source.