Molecular and Cellular Effects of Heat-shock and Related Treatments of Mammalian Tissue-culture Cells
This extract was created in the absence of an abstract.
Excerpt
Most, if not all, organisms, from prokaryotes to the most complex eukaryotes, exhibit specific homeostatic-type responses when their environmental circumstance is perturbed. Such responses range from temporary and reversible modifications of enzyme function, by which specific metabolic pathways are redirected or arrested, to more long-term changes, which may involve altered patterns of gene expression of a more permanent nature. The subject of this paper is the induction of a particular set of genes in cultured mammalian cells in response to growth at elevated temperatures or in medium containing amino acid analogs. These treatments (as well as a variety of others) give rise to what we refer to as the stress response, and for heat shock there is evidence that the response has a protective effect on cells (Gerner et al. 1976; McAllister and Finkelstein 1980; Petersen and Mitchell 1981).
STRESS RESPONSE
In many organisms, as well as in cells grown...
