Studies on In Vitro Transformation by DNA and DNA Fragments of Human Adenoviruses and Simian Virus 40
- F. L. Graham,
- P. J. Abrahams,
- C. Mulder*,
- H. L. Heijneker†,
- S. O. Warnaar,
- F. A. J. de Vries,
- W. Fiers**, and
- A. J. van der Eb
- Laboratory for Physiological Chemistry, University of Leiden, Leiden, The Netherlands
- †Laboratory of Molecular Genetics, University of Leiden, Leiden, The Netherlands
- *Cold Spring Harbor Laboratory, Cold Spring Harbor, New York 11724
- **Laboratory for Molecular Biology, University of Ghent, Ghent, Belgium
This extract was created in the absence of an abstract.
Excerpt
There is considerable evidence suggesting that only part of the genome of the DNA tumor viruses is involved in oncogenic transformation: (1) The transforming activity of these viruses is much more resistant to inactivation by radiation and chemicals than is the infectivity, indicating that a considerable part of the viral genome is not required for transformation (Benjamin 1965; Yamamoto 1970). (2) Usually only the early viral gene functions are expressed in transformed cells (Ozanne et al. 1973; Green et al. 1970). (3) Studies with temperature-sensitive mutants have shown that late mutants are normal with respect to transformation at the restrictive temperature, and there is evidence that in the case of polyoma virus and simian virus 40 (SV40) only one or two gene functions are required for transformation (Eckhart 1969; Eckhart et al. 1971; Tegtmeyer and Ozer 1971). (4) Cells transformed by adenovirus type 2 contain only a part of the...
