Cleavage Maps of DNA from Adenovirus Types 2 and 5 by Restriction Endonucleases EcoRI and HpaI
This extract was created in the absence of an abstract.
Excerpt
The genomes of the human adenoviruses sero-types 2 and 5 (Ad2 and Ad5) are linear duplex DNA molecules with molecular weights of 23 × 106 (Green et al. 1967; van der Eb et al. 1969) whose structure has been studied by cleavage with site-specific endonucleases.
Endonuclease R·EcoRI (Yoshimori 1971) cleaves Ad2 DNA into six and Ad5 DNA into three unique fragments (Pettersson et al. 1973; Mulder et al. 1974). Endonuclease R·HpaI cleaves both Ad2 DNA (Sharp et al. 1973) and Ad5 DNA into seven unique fragments. These fragments are being used for various studies on the replication, transcription and translation of Ad2 and Ad5 DNA and on cell transformation by these genomes. In order to use the fragments for these studies, it was necessary to establish the location of the fragments in the intact molecule.
The fragments of Ad2 and Ad5 DNA produced by endonucleases R-EcoRI and HpaI have been...
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↵* Present address: The Wallenberg Laboratory, Department of Microbiology, Uppsala, Sweden.
