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Volume 143, Issue 4

Copper-inducible transcriptional regulation at two promoters in the copper resistance determinant Free

Abstract

The determinant of plasmid pRJ1004 encodes inducible resistance to the trace element copper. The identification of two copper-dependent transcriptional initiation regions within that each contain a similar upstream hyphenated dyad motif is described. Deletion constructs showed that this “copper box” motif was essential for copper-inducible activity at both pco promoters, P and P The placement of the motif differs in the two promoters, and P contains an extended -10 nonamer typical of promoters for which RNA polymerase does not bind specifically to -35 sequences. P does not contain this motif and is the more strongly expressed promoter. The transcript from P contains the genes, while expresses only The induction profiles for and P fusions were flattened sigmoidal curves with a gradual response to increasing copper concentration. On high-copy-number plasmids, zinc was found also to induce transcription from both promoters Both promoters showed inducible activity in the absence of the plasmid-borne two-component regulatory system, indicating that a second trans-acting regulatory system is present on the chromosome. The product showed repressor action in the absence of while still allowing induction, suggesting the chromosome encoded a similar two-component system to Tn insertion mutagenesis identified chromosomal genes which affected promoter expression, including (sugar phosphotransferase system) and cya (adenylate cyclase). The results support that idea that -encoded copper resistance is an auxiliary mechanism for handling copper, the regulation of which is integrated with the chromosomal regulation of cellular copper metabolism.

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Keyword(s): copper resistance , gene regulation , homeostasis , plasmid and trace element
© Society for General Microbiology 1997
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1997-04-01
2024-04-19
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Copper-inducible transcriptional regulation at two promoters in the Escherichia coli copper resistance determinant pco
Microbiology 143, 1191 (1997); https://doi.org/10.1099/00221287-143-4-1191
/content/journal/micro/10.1099/00221287-143-4-1191
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