1887

Abstract

66 was shown to harbour two self-transmissible plasmids: SLP2, which acts as a sex factor, and SLP3. Derivatives of this strain which had lost both plasmids were used as host strains to study a range of plasmids for their ability to promote their own transfer and to mobilize chromosomal markers. A linkage map of the chromosome containing ten markers was derived from the results of matings using several different sex plasmids, and protoplast fusions. SLP2 was transferred interspecifically to ATCC 12434 and to A3(2); in the latter it acted as a fertility factor. Interspecific crosses also led to the discovery of a further plasmid, SLP4, from SLP2, SLP3 and SLP4 could not be visualized on agarose gels using standard plasmid isolation procedures, but their presence was detected by transformation into

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-129-7-2257
1983-07-01
2024-04-19
Loading full text...

Full text loading...

/deliver/fulltext/micro/129/7/mic-129-7-2257.html?itemId=/content/journal/micro/10.1099/00221287-129-7-2257&mimeType=html&fmt=ahah

References

  1. Baltz R.H. 1980; Genetic recombination by protoplast fusion inStreptomyces. Developments in Industrial Microbiology 21:43–54
    [Google Scholar]
  2. Bibb M.J, Hopwood D.A. 1981; Genetic studies of the fertility plasmid SCP2 and its SCP2* variants inStreptomyces coelicolor A3(2). Journal of General Microbiology 126:427–442
    [Google Scholar]
  3. Bibb M.J, Freeman R.F, Hopwood D.A. 1977; Physical and genetical characterisation of a second sex factor, SCP2, forStreptomyces coelicolor A 3(2). Molecular and General Genetics 154:155–166
    [Google Scholar]
  4. Bibb M.J, Ward J.M, Kieser T, Cohen S.N, Hopwood D.A. 1981; Excision of chromosomal DN A sequences fromStreptomyces coelicolor forms a novel family of plasmids detectable inStreptomyces lividans. Molecular and General Genetics 184:230–240
    [Google Scholar]
  5. Bibb M.J, Chater K.F, Hopwood D.A. 1983; Developments in Streptomyces cloning. In Experimental Manipulation of Gene Expression Edited by Inouye M. New York: Academic Press (in the Press);
    [Google Scholar]
  6. Chater K.F, Hopwood D.A. 1983; Streptomyces genetics. In Biology of the Actinomycetes Edited by Goodfellow M., Mordarski M., Williams S. T. London: Academic Press (in the Press);
    [Google Scholar]
  7. Chater K.F, Hopwood D.A, Kieser T, Thompson C.J. 1982; Gene cloning inStreptomyces.. Current Topics in Microbiology and Immunology 96:69–95
    [Google Scholar]
  8. Freeman R. F., Bibb M.J, Hopwood D.A. 1977; Chloramphenicol acetyltransferase-independent chloramphenicol resistance inStreptomyces coelicolor A3(2). Journal of General Microbiology 98:453–465
    [Google Scholar]
  9. Friend E.J, Hopwood D.A. 1971; The linkage map ofStreptomyces rimosus. Journal of General Microbiology 68:187–197
    [Google Scholar]
  10. Hoopes B.C, Mcclure W.R. 1981; Studies on the selectivity of DNA precipitation by spermine. Nucleic Acids Research 9:5493–5504
    [Google Scholar]
  11. Hopwood D.A. 1959; Linkage and the mechanism of recombination inStreptomyces coelicolor. Annals of the New York Academy of Sciences 81:887–898
    [Google Scholar]
  12. Hopwood D.A. 1966; Lack of constant genome ends inStreptomyces coelicolor. Genetics 54:1177–1184
    [Google Scholar]
  13. Hopwood D.A. 1967; Genetic analysis and genome structure inStreptomyces coelicolor. Bacteriological Reviews 31:373–403
    [Google Scholar]
  14. Hopwood D.A. 1972; Genetic analysis in microorganisms. Methods in Microbiology 7B:29–158
    [Google Scholar]
  15. Hopwood D.A. 1981a; Uses of protoplasts in the genetic manipulation of streptomycetes. Zentralblatt für Bakteriologie Suppl 11:523–531
    [Google Scholar]
  16. Hopwood D.A. 1981b; Genetic studies with bacterial protoplasts. Annual Review of Microbiology 35:237–272
    [Google Scholar]
  17. Hopwood D.A, Chater K.F. 1982; Cloning inStreptomyces: systems and strategies. In Genetic Engineering 4 pp 119–145 Edited by Setlow J.K, Hollaender A. New York: Plenum;
    [Google Scholar]
  18. Hopwood D.A, Wright H.M. 1973; A plasmid ofStreptomyces coelicolor carrying a chromosomal locus and its inter-specific transfer. Journal of General Microbiology 79:331–342
    [Google Scholar]
  19. Hopwood D.A, Wright H.M. 1978; Bacterial protoplast fusion: recombination in fused protoplasts ofStreptomyces coelicolor. Molecular and General Genetics 162:307–317
    [Google Scholar]
  20. Hopwood D.A, Chater K.F, Dowding J.E, Vivian A. 1973; Advances inStreptomyces coelicolor genetics. Bacteriological Reviews 37:371–405
    [Google Scholar]
  21. Hopwood D.A, Wright H.M, Bibb M.J, Cohen S.N. 1977; Genetic recombination through protoplast fusion inStreptomyces. Nature, London 268:171–174
    [Google Scholar]
  22. Kieser T, Hopwood D.A, Wright H.M, Thompson C.J. 1982; pIJlOl, a multicopy broad host-rangeStreptomyces plasmid: functional analysis and development of DNA cloning vectors. Molecular and General Genetics 185:223–238
    [Google Scholar]
  23. Kirby R. 1976 Genetic studies on Streptomyces coelicolor plasmid one Ph.D. thesis University of East Anglia; Norwich, U.K:
    [Google Scholar]
  24. Okanishi M, Suzuki K, Umezawa H. 1974; Formation and reversion of streptomycete protoplasts : cultural conditions and morphological study. Journal of General Microbiology 80:389–400
    [Google Scholar]
  25. Schrempf H, Goebel W. 1977; Characterisation of a plasmid fromStreptomyces coelicolor A3(2). Journal of Bacteriology 131:251–258
    [Google Scholar]
  26. Thompson C.J, Ward J.M, Hopwood D.A. 1980; DNA cloning inStreptomyces: resistance genes from antibiotic producing species. Nature, London 286:527–529
    [Google Scholar]
  27. Thompson C.J, Ward J.M, Hopwood D.A. 1982a; Cloning of antibiotic resistance and nutritional genes in streptomycetes. Journal of Bacteriology 151:668–677
    [Google Scholar]
  28. Thompson C.J, Kieser T, Ward J.M, Hopwood D.A. 1982b; Physical analysis of antibiotic resistance genes fromStreptomyces and their use in vector construction. Gene 20:51–62
    [Google Scholar]
  29. Vivian A. 1971; Genetic control of fertility inStreptomyces coelicolor A3(2): plasmid involvement in the interconversion of UF and IF strains. Journal of General Microbiology 69:353–364
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-129-7-2257
Loading
/content/journal/micro/10.1099/00221287-129-7-2257
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error