Europe PMC

This website requires cookies, and the limited processing of your personal data in order to function. By using the site you are agreeing to this as outlined in our privacy notice and cookie policy.

Viral pathogenicity determinants are suppressors of transgene silencing in Nicotiana benthamiana.

Author information

Affiliations

  • 1. The Sainsbury Laboratory, John Innes Centre, Colney Lane, Norwich NR4 7UH, UK.
      Authors
    • Brigneti G 1
    (1 author)

ORCIDs linked to this article

The EMBO Journal, 01 Nov 1998, 17(22):6739-6746
https://doi.org/10.1093/emboj/17.22.6739 PMID: 9822616 PMCID: PMC1171019

Free full text in Europe PMC

This article has been retracted. See Brigneti G, Voinnet O, Li WX, Ji LH, Ding SW, Baulcombe DC. EMBO J. 2015 Oct 14;34(20):2595.

Abstract 

Post-transcriptional gene silencing (PTGS) of a green fluorescent protein (GFP) transgene is suppressed in Nicotiana benthamiana plants infected with potato virus Y (PVY) or with cucumber mosaic virus (CMV), but not in plants infected with potato virus X (PVX). By expressing PVY and CMV-encoded proteins in a PVX vector we have shown that the viral suppressors of gene silencing are the HCPro of PVY and the 2b protein of CMV. The HCPro acts by blocking the maintenance of PTGS in tissues where silencing had already been set, whereas the 2b protein prevents initiation of gene silencing at the growing points of the plants. Combined with previous findings that viruses are both activators and targets of PTGS, these data provide compelling evidence that PTGS represents a natural mechanism for plant protection against viruses.

Free full text 

Logo of embojLink to Publisher's site
EMBO J. 1998 Nov 16; 17(22): 6739–6746.
PMCID: PMC1171019
PMID: 9822616

This article has been retracted. Retraction in: EMBO J. 2015 October 14; 34(20): 2595    See also: PMC Retraction Policy

Viral pathogenicity determinants are suppressors of transgene silencing in Nicotiana benthamiana.

G Brigneti, O Voinnet, W X Li, L H Ji, S W Ding, and D C Baulcombe
Author information Copyright and License information Disclaimer

Abstract

Post-transcriptional gene silencing (PTGS) of a green fluorescent protein (GFP) transgene is suppressed in Nicotiana benthamiana plants infected with potato virus Y (PVY) or with cucumber mosaic virus (CMV), but not in plants infected with potato virus X (PVX). By expressing PVY and CMV-encoded proteins in a PVX vector we have shown that the viral suppressors of gene silencing are the HCPro of PVY and the 2b protein of CMV. The HCPro acts by blocking the maintenance of PTGS in tissues where silencing had already been set, whereas the 2b protein prevents initiation of gene silencing at the growing points of the plants. Combined with previous findings that viruses are both activators and targets of PTGS, these data provide compelling evidence that PTGS represents a natural mechanism for plant protection against viruses.

Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Baulcombe DC. Mechanisms of Pathogen-Derived Resistance to Viruses in Transgenic Plants. Plant Cell. 1996 Oct;8(10):1833–1844. [Abstract] [Google Scholar]
  • Baulcombe DC, Chapman S, Santa Cruz S. Jellyfish green fluorescent protein as a reporter for virus infections. Plant J. 1995 Jun;7(6):1045–1053. [Abstract] [Google Scholar]
  • Chapman S, Kavanagh T, Baulcombe D. Potato virus X as a vector for gene expression in plants. Plant J. 1992 Jul;2(4):549–557. [Abstract] [Google Scholar]
  • Cronin S, Verchot J, Haldeman-Cahill R, Schaad MC, Carrington JC. Long-distance movement factor: a transport function of the potyvirus helper component proteinase. Plant Cell. 1995 May;7(5):549–559. [Abstract] [Google Scholar]
  • Depicker A, Montagu MV. Post-transcriptional gene silencing in plants. Curr Opin Cell Biol. 1997 Jun;9(3):373–382. [Abstract] [Google Scholar]
  • Ding SW, Li WX, Symons RH. A novel naturally occurring hybrid gene encoded by a plant RNA virus facilitates long distance virus movement. EMBO J. 1995 Dec 1;14(23):5762–5772. [Europe PMC free article] [Abstract] [Google Scholar]
  • English JJ, Mueller E, Baulcombe DC. Suppression of Virus Accumulation in Transgenic Plants Exhibiting Silencing of Nuclear Genes. Plant Cell. 1996 Feb;8(2):179–188. [Abstract] [Google Scholar]
  • Fire A, Xu S, Montgomery MK, Kostas SA, Driver SE, Mello CC. Potent and specific genetic interference by double-stranded RNA in Caenorhabditis elegans. Nature. 1998 Feb 19;391(6669):806–811. [Abstract] [Google Scholar]
  • Hamilton CM, Frary A, Lewis C, Tanksley SD. Stable transfer of intact high molecular weight DNA into plant chromosomes. Proc Natl Acad Sci U S A. 1996 Sep 3;93(18):9975–9979. [Europe PMC free article] [Abstract] [Google Scholar]
  • Kavanagh T, Goulden M, Santa Cruz S, Chapman S, Barker I, Baulcombe D. Molecular analysis of a resistance-breaking strain of potato virus X. Virology. 1992 Aug;189(2):609–617. [Abstract] [Google Scholar]
  • Kjemtrup S, Sampson KS, Peele CG, Nguyen LV, Conkling MA, Thompson WF, Robertson D. Gene silencing from plant DNA carried by a Geminivirus. Plant J. 1998 Apr;14(1):91–100. [Abstract] [Google Scholar]
  • Kumagai MH, Donson J, della-Cioppa G, Harvey D, Hanley K, Grill LK. Cytoplasmic inhibition of carotenoid biosynthesis with virus-derived RNA. Proc Natl Acad Sci U S A. 1995 Feb 28;92(5):1679–1683. [Europe PMC free article] [Abstract] [Google Scholar]
  • Lindbo JA, Silva-Rosales L, Proebsting WM, Dougherty WG. Induction of a Highly Specific Antiviral State in Transgenic Plants: Implications for Regulation of Gene Expression and Virus Resistance. Plant Cell. 1993 Dec;5(12):1749–1759. [Abstract] [Google Scholar]
  • Palauqui JC, Elmayan T, Pollien JM, Vaucheret H. Systemic acquired silencing: transgene-specific post-transcriptional silencing is transmitted by grafting from silenced stocks to non-silenced scions. EMBO J. 1997 Aug 1;16(15):4738–4745. [Europe PMC free article] [Abstract] [Google Scholar]
  • Pruss G, Ge X, Shi XM, Carrington JC, Bowman Vance V. Plant viral synergism: the potyviral genome encodes a broad-range pathogenicity enhancer that transactivates replication of heterologous viruses. Plant Cell. 1997 Jun;9(6):859–868. [Abstract] [Google Scholar]
  • Robaglia C, Durand-Tardif M, Tronchet M, Boudazin G, Astier-Manifacier S, Casse-Delbart F. Nucleotide sequence of potato virus Y (N Strain) genomic RNA. J Gen Virol. 1989 Apr;70(Pt 4):935–947. [Abstract] [Google Scholar]
  • Ruiz F, Vayssié L, Klotz C, Sperling L, Madeddu L. Homology-dependent gene silencing in Paramecium. Mol Biol Cell. 1998 Apr;9(4):931–943. [Europe PMC free article] [Abstract] [Google Scholar]
  • Ruiz MT, Voinnet O, Baulcombe DC. Initiation and maintenance of virus-induced gene silencing . Plant Cell. 1998 Jun;10(6):937–946. [Abstract] [Google Scholar]
  • Smith HA, Swaney SL, Parks TD, Wernsman EA, Dougherty WG. Transgenic plant virus resistance mediated by untranslatable sense RNAs: expression, regulation, and fate of nonessential RNAs. Plant Cell. 1994 Oct;6(10):1441–1453. [Abstract] [Google Scholar]
  • Thole V, Dalmay T, Burgyán J, Balázs E. Cloning and sequencing of potato virus Y (Hungarian isolate) genomic RNA. Gene. 1993 Jan 30;123(2):149–156. [Abstract] [Google Scholar]
  • Vance VB. Replication of potato virus X RNA is altered in coinfections with potato virus Y. Virology. 1991 Jun;182(2):486–494. [Abstract] [Google Scholar]
  • Voinnet O, Baulcombe DC. Systemic signalling in gene silencing. Nature. 1997 Oct 9;389(6651):553–553. [Abstract] [Google Scholar]
Articles from The EMBO Journal are provided here courtesy of Nature Publishing Group

Full text links 

Read article at publisher's site: https://doi.org/10.1093/emboj/17.22.6739

Read article for free, from open access legal sources, via Unpaywall: https://europepmc.org/articles/pmc1171019?pdf=renderUnpaywall

Citations & impact 

Impact metrics

587
Citations
Jump to Citations
1
Data citation
Jump to Data

Citations of article over time

Alternative metrics

Altmetric item for https://www.altmetric.com/details/3036898
Altmetric
Discover the attention surrounding your research
https://www.altmetric.com/details/3036898

Smart citations by scite.ai
Smart citations by scite.ai include citation statements extracted from the full text of the citing article. The number of the statements may be higher than the number of citations provided by EuropePMC if one paper cites another multiple times or lower if scite has not yet processed some of the citing articles.
Explore citation contexts and check if this article has been supported or disputed.
https://scite.ai/reports/10.1093/emboj/17.22.6739

Supporting
Mentioning
Contrasting
30
680
0

Article citations

Go to all (587) article citations

Data 

Similar Articles 

To arrive at the top five similar articles we use a word-weighted algorithm to compare words from the Title and Abstract of each citation.