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Kiyoshi Ameno, Chiaki Fuke, Setsuko Ameno, Takahiro Kiriu, Toyohiko Shinohara, Iwao Ijiri, A Rapid and Sensitive Quantitation of Amitraz in Plasma by Gas Chromatography with Nitrogen-Phosphorus Detection and Its Application for Pharmacokinetics, Journal of Analytical Toxicology, Volume 15, Issue 3, May-June 1991, Pages 116–118, https://doi.org/10.1093/jat/15.3.116
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Abstract
We report a simple, sensitive, and rapid quantitation of Amitraz in plasma after Extrelut-3 column extraction by gas chromatography with nitrogen—phosphorus detection (GC-NPD). The plasma sample was diluted four-fold with borate buffer (0.01 M, pH 11), put into an Extrelut-3 column, left for 15 min, and then eluted with 15 mL of n-hexane. The n-hexane eluate was evaporated under nitrogen gas flow at room temperature. The residue was reconstituted with 0.1 mL of acetone containing nitrazepam as an internal standard. A 2-µL aliquot was injected into a wide-bore capillary column GC-NPD. The detection limit was 0.5 ng/mL and linearity was obtained in the range of 1–200 ng/mL. Amitraz in the buffer at pH 11 remained stable in a freezer for one week at −20°C. The GC-NPD method was found useful in studying the pharmacokinetics of a single dose intravenous administration of Amitraz to a dog.