Summary
An efficient and reproducible procedure for the large-scale propagation of Pseudoxytenanthera stocksii is described. High-frequency multiple shoot induction was achieved from nodal shoot segments collected from superior/elite genotypes on Murashige and Skoog (MS) liquid medium supplemented with 1-naphthaleneacetic acid (NAA; 2.68 μM) and 6-benzylaminopurine (BA; 4.40 μM) at 28±1°C and 60 μmol m−2 s−1 light intensity under 12h photoperiod. In vitro-differentiated shoots were multiplied on MS liquid medium fortified with NAA (2.68 μM), BA (2.21 μM) and additives: ascorbic acid (283.93 μM), citric acid (118.10 μM), cysteine (104.04 μM), and glutamine (342.24 μM). Subculturing was carried out every 2wk on fresh shoot multiplication medium. About 125–150 shoots per culture flask were harvested within 45–50d. In vitro-differentiated shoot clumps (three or four shoots) were successfully rooted on half-strength MS basal liquid medium with indole-3-butyric acid (4.90 μM), BA (0.44 μM), and additives. This is the first report where in vitro- and in vivo-(through tillers) raised clonal plants were acclimatized and established in the field, where they exhibited normal growth.
Similar content being viewed by others
References
Anil, S.; Ahuja, P. S.; Sharma, M.; Sharma, O. P.; Godbole, S. In vitro protocols and field performance of elites of an important bamboo Dendrocalamus hamiltonii Nee et Arn. Munro. Plant Cell Tiss. Organ Cult. 71:55–63; 2002.
Bag, N.; Chandra, S.; Palni, L. M. S.; Nandi, S. K. Micropropagation of Devringal (Thamnocalamus spathiflorus (Trin.) Munro)—a temperate bamboo, and comparison between in vitro propagated plants and seedlings. Plant Sci. 156:125–135; 2000.
Bystriakova, N.; Kapos, V.; Lysenko, I.; Stapleton, C. M. A. Distribution and conservation status of forest bamboo biodiversity in the Asia-Pacific region. Biodiversity Conserv. 12:1833–1841; 2003.
Chang, W. C.; Ho, C. W. Micropropagation of bamboos. In: Bajaj, Y. P. S., ed. Biotechnology in agriculture and forestry, high-tech and micropropagation V. Berlin: Springer-Verlag; 1997:203–219.
Chaturvedi, H. C.; Sharma, M.; Sharma, A. K. In vitro regeneration of Dendrocalamus strictus Nees. through nodal segments taken from field grown culms. Plant Sci. 91:97–101; 1993.
Lin, C. S.; Chang, W. C. Micropropagation of Bambusa edulis through nodal explants of field grown culms and flowering of regenerated plantlets. Plant Cell Rep. 17:617–620; 1998.
Lin, C. S.; Lin, C. C.; Chang, W. C. Effect of thidiazuron on vegetative tissue derived somatic embryogenesis and flowering of bamboo Bambusa edulis. Plant Cell Tiss. Organ Cult. 75:75–82; 2004.
Mukunthakumar, S.; Mathur, J.; Nair, P. K. K.; Mathur, S. N. Micropropagation of Dendrocalamus brandisii Kurz. using in vitro nodal explants. Indian Forester 12:1239–1243; 1999.
Murashige, T.; Skoog, F. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant. 15:473–497; 1962.
Nadgauda, R. S.; Parassharami, V. A.; Mascarenhas, A. S. Precocious flowering and seedling behaviour in tissue cultured bamboos. Nature 344:335–336; 1990.
Prutpongse, P.; Gayinlertvatana, P. In vitro micropropagation of 54 species from 15 genera of bamboo. HortScience 27:453–458; 1992.
Ramanayake, S. M. S. D.; Wanniarachchi, W. A. V. R. Organogenesis in callus derived from an adult giant bamboo (Dendrocalamus giganteus Wall. Ex Munro). Sci. Hort. 98:195–200; 2003.
Ramanayake, S. M. S. D.; Yakandawala, K. Micropropagation of the giant bamboo (Dendrocalamus giganteus Munro) from nodal explants of field grown culms. Plant Sci. 129:213–223; 1997.
Ravikumar, R. G.; Ananthakrishnan, K.; Kathiravan, K.; Ganapathi, A. In vitro shoot propagation of Dendrocalamus strictus nees. Plant Cell Tiss. Organ Cult. 52:189–192; 1998.
Reddy, Y.; Yekanthappa, A. N. Propagation technique of Oxytenanthera stocksii. Myforest 25:30–32; 1989.
Saxena, S.; Bhojwani, S. S. In vitro clonal multiplication of 4-year-old plants of the bamboo, Dendrocalamus longispathus Kurz. In Vitro Cell. Dev. Biol. Plant 29:135–142; 1993.
Tewari, D. N. A monograph on bamboo. Dehra Dun, India: International Book Distibutors; 1992:401–498.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Sanjaya, Rathore, T.S. & Ravishankar Rai, V. Micropropagation of Pseudoxytenanthera stocksii Munro. In Vitro Cell.Dev.Biol.-Plant 41, 333–337 (2005). https://doi.org/10.1079/IVP2004625
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1079/IVP2004625