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A simple wheat haploid and doubled haploid production system using anther culture

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Summary

Wheat (Triticum aestivum L.) haploids and doubled haploids have been used in breeding programs and genetic studies. Wheat haploids and doubled haploids via anther culture are usually produced by a multiple step culture procedure. We improved a wheat haploid and doubled haploid production system via anther culture in which plants are produced from microspore-derived embryos using one medium and one culture environment. In the improved protocol, tillers of donor plants were pretreated at 4°C for 1–2 wk before anthers were plated on a modified 85D12 basal medium with phenylacetic acid (PAA) and zeatin and cultured at 30°C with a 12-h daylength (43 μEs−1m−2) in an incubator. Microspore-derived embryos developed in 2–3 wk and the plants were produced 3–4 wk after anther plating. In the improved system, as much as 53% of the anthers of Pavon 76 were responsive with multiple embryos. For plant regeneration, as many as 22 green and 25 albino plants were produced from 100 anthers. Sixty-five green plants were grown to maturity and 32 (49%) plants were fertile and produced seeds (indicating spontaneous chromosome doubling) while 33 plants did not produce seed. Of five Nebraska breeding lines tested using the protocol, NE96675 was very responsive and the other lines less so, indicating that the protocol is genotype-dependent.

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Authors and Affiliations

  1. Kumho Life & Environmental Science Laboratory (KLESL), 500-712, Gwangju, South Korea

    Kyung-Moon Kim

  2. Department of Agronomy and Horticulture, University of Nebraska, 68583-0915, Lincoln, NE

    P. Stephen Baenziger

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  1. Kyung-Moon Kim
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  2. P. Stephen Baenziger
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Correspondence to P. Stephen Baenziger.

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Kim, KM., Baenziger, P.S. A simple wheat haploid and doubled haploid production system using anther culture. In Vitro Cell.Dev.Biol.-Plant 41, 22–27 (2005). https://doi.org/10.1079/IVP2004594

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  • DOI: https://doi.org/10.1079/IVP2004594

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