Response of seedlings to heat-stress in cultivars of wheat: Growth temperature-dependent differential modulation of photosystem 1 and 2 activity, and foliar antioxidant defense capacity

doi:10.1078/0176-1617-00594

Journal of Plant Physiology

Volume 159, Issue 1, 2002, Pages 49-59

https://doi.org/10.1078/0176-1617-00594 Get rights and content

Summary

Impact of elevated temperature on the development of photosystems and foliar antioxidant activity in greening seedlings was examined for characterizing the response of wheat (Triticum aestivum L.) cultivars to heat-stress. Greening of seedlings for 60 h at temperatures above 25 °C induced alterations in photosystem (PS) 1 and 2 activities differentially in chloroplasts isolated from developing primary leaves. The electron flow driven by PS1 was stimulated in contrast to sharp inhibition of that mediated by PS2. Compared to HD1553, HD2329, and C306, heat-stressed seedlings of HD2307 had higher percentage of functional photosystem 2. In order to characterize the differential stability of both photosystems and the role of the antioxidant defense system, if any, in response to heat-stress among cultivars, the activity of superoxide dismutase, catalase, ascorbate peroxidase, and glutathione reductase enzymes associated with ascorbate-glutathione recycling and scavenging of H₂O₂ was analyzed. Heat-stress during greening led to stimulation in foliar superoxide dismutase activity, indicating a potential source for generation of its substrate, superoxide radical (O⁻₂), in light. Ascorbate peroxidase, a key H₂O₂-metabolizing enzyme, was stimulated differentially within cultivars and maximally in HD2307. Moreover, the efficiency of this enzyme exhibited a linear rise with increase in growth temperature. H₂O₂-utilizing catalase enzyme was also stimulated in seedlings greened at non-lethal temperatures of 30 and 35 °C, but was inactivated markedly in 40 °C-stressed seedlings of different cultivars, except HD2307. The level of foliar ascorbate and glutathione increased in response to greening at elevated temperatures in cultivars, albeit markedly in HD2307. In summary, heat-stress during greening led to inactivation of PS2 vis-à-vis highturn over of both PS1 and superoxide dismutase, which allowed formation of superoxides and its dismutation product, H₂O₂, and reversible modulation of enzymatic H₂O₂-scavenging. This suggests a central role of H₂O₂ metabolism in seedling response to thermal stress. Heat-tolerance potential of cultivars, evident in HD2307 seedlings, co-relates to the potential of ascorbate peroxidase and catalase to dissipate H₂O₂ co-operatively and ameliorate photo-oxidative damage to heat-stressed seedlings. Ascorbate peroxidase, because of its temperature-dependent linear activation and stability over a broad range of elevated temperatures, is suitable for introducing tolerance to heat-stress in sensitive crops. This study signifies the importance of antioxidant evaluation in selection of tolerant lines as well as the role of H₂O₂ in heat-stress response of seedlings.

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Response of seedlings to heat-stress in cultivars of wheat: Growth temperature-dependent differential modulation of photosystem 1 and 2 activity, and foliar antioxidant defense capacity

Summary

J Plant Physiol

Analyt Biochem

Anal Biochem

J Plant Physiol

Biochem Biophys Acta

Chem Phys Lipids

J Plant Physiol

Differentiation

J Plant Physiol

Eur J Agron

J Plant Physiol

Analytical Biochem

J Plant Physiol

Catalase

Photosynthesis and productivity during high temperature stress of wheat genotypes from major world regions

Crop Sci

The photoproduction of superoxide radicals and the superoxide dismutase activity of photosystem II. The possible involvement of cytochrome b559

Photosynth Res

Radical producion and scavenging in chloroplasts

Active oxygen mediates heat-indued resistance of rice plants to blast disease

Plant Sci

Alterations in the active oxygen scavenging enzymes of wheat leaves subjected to water stress

Plant Cell Physiol

Effect of high-temperature stress on the photosynthetic apparatus

Hydrogen peroxide metabolism as an index of water stress tolerance in jute

Physiol Plant

Parallel changes in H2O2 and catalase during thermotolerance induced by salicylic acid or heat-acclimation in mustard seedlings

Plant Physiol

Heat-shock responses of bean plants: involvement of free radicals, antioxidants and free radical/active oxygen scavenging systems

Biol Plant

Photoinactivation of catalase occurs under both high and low temperature stress conditions and accompanies photoinhibition of photosystem II

Plant Physiol

Oxygen processing in photosynthesis: regulation and signalling

New Phytol

Photooxidative stress in plants

Physiol Plant

Hydrogen peroxide- and glutathione-associated mechanisms of acclimatory stress tolerance and signalling

Physiol Plant

Over expression of glutathione reductase but not glutathione synthase leads to increases in antioxidant capacity and improved photosynthesis in poplar tress

Plant Physiol

Effects of water stress on the chloroplast antioxidant system. I. Alterations in glutathione reductase activity

Plant Physiol

Chloroplast protection in greening leaves

Physiol Plant

Role of reactive oxygen intermediates and cognate redox signaling in disease resistance

Plant Physiol

Cis-elements and trans-factors that regulate expression of the maize Cat1 antioxidant gene in response to ABA and osmotic stress: H2O2 is the likely intermediary signaling molecule for the response

Plant J

Parallel changes in H₂O₂ and catalase during thermotolerance induced by salicylic acid or heat-acclimation in mustard seedlings

Cis-elements and trans-factors that regulate expression of the maize Cat1 antioxidant gene in response to ABA and osmotic stress: H₂O₂ is the likely intermediary signaling molecule for the response