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Horm Metab Res 2008; 40(3): 219-224
DOI: 10.1055/s-2008-1046793
Humans, Clinical

© Georg Thieme Verlag KG Stuttgart · New York

Association of the 163A/G and 1181G/C Osteoprotegerin Polymorphism with Bone Mineral Density

M. T. García-Unzueta 1 , J. A. Riancho 2 , M. T. Zarrabeitia 3 , C. Sañudo 2 , A. Berja 4 , C. Valero 2 , C. Pesquera 4 , B. Paule 4 , J. González-Macías 2 , J. A. Amado 4
  • 1Clinical Biochemistry, Hospital Universitario “Marqués de Valdecilla”, University of Cantabria, Santander, Spain
  • 2Internal Medicine, Hospital Universitario “Marqués de Valdecilla”, University of Cantabria, Santander, Spain
  • 3Legal Medicine, University of Cantabria, Santander, Spain
  • 4Endocrinology Services, Hospital Universitario “Marqués de Valdecilla”, University of Cantabria, Santander, Spain
Further Information

Publication History

received 22.03.2007

accepted 14.08.2007

Publication Date:
17 March 2008 (online)

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Abstract

The aim of the study was to investigate the distribution of 163 A/G osteoprotegerin gene promoter and 1181 G/C osteoprotegerin exon 1 polymorphisms in a group of women with different hormonal status and to analyze their relationship with BMD. Osteoprotegerin polymorphisms and BMD were analyzed in 332 women (69 premenopausal and 263 postmenopausal). BMD was quantified at the lumbar spine (L2-4), femoral neck, and total hip. Genotyping for the presence of different polymorphisms was performed using the Custom Taqman® SNP Genotyping assays. There were not significant differences in BMD according to 163 A/G genotype. However, significant differences in lumbar spine BMD were found according to 1181 G/C alleles. Thus, women with CC genotype had significant higher BMD at the lumbar spine than those with GC or GG genotype. No differences were found in femoral neck and total hip BMD. In age-adjusted models, the 1181 G/C OPG polymorphism explained 2.2% of BMD variance at the spine, 0.3% at the femoral neck, and 0.9% at the total hip in the whole group. In the subgroup of premenopausal women, the polymorphism was strongly related to spine BMD, and explained 11.5% of the variance, whereas body weight explained 7.9%. The 1181 G/C polymorphism was associated with lumbar spine BMD in Spanish women. Premenopausal women with the CC genotype had a higher BMD.

Key words

osteoprotegerin - single nucleotide polymorphisms - 1181 G/C - 163 A/G - bone mineral density

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Correspondence

M. T. García-Unzueta

Clinical Biochemistry

Hospital Universitario “Marqués de Valdecilla”

University of Cantabria

Valdecilla Street

39008 Santander

Spain

Phone: +34/942/20 25 14

Fax: +34/942/20 16 95, +34/942/20 16 63

Email: mgunzueta@humv.es

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