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Horm Metab Res 1980; 12(4): 133-135
DOI: 10.1055/s-2007-996224
ORIGINALS

© Georg Thieme Verlag, Stuttgart · New York

Glucose Clamping Using the Biostator GCIIS

C. A. Verdonk, R. A. Rizza, R. E. Westland, R. L. Nelson, J. E. Gerich, F. J. Service
  • Division of Endocrinology/Metabolism and Internal Medicine, Mayo Clinic and Mayo Foundation, Rochester, Minnesota, U.S.A.
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Publication History

1979

1979

Publication Date:
14 March 2008 (online)

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Summary

The Biostator GCIIS was used to clamp circulating glucose at hypoglycemic (42 ± 1 mg/dl), euglycemic (86 ± 2 mg/dl), and hyperglycemic (142 ± 1 mg/dl) levels in normal subjects during a concomitant infusion of insulin (0.1 U/kg/h). Because of limitations in maximal glucosè infusion (1 g/min) from the Biostator, a supplementary infusion of glucose was required to accomplish euglycemic and hyperglycemic clamps. The coefficients of variation of blood glucose were 7.06 ± 1.3 %, 5.9 ± 0.5 %, and 6.1 ± 0.9 for 120 minutes of hypoglycemic, euglycemic, and hyperglycemic clamps, respectively. Despite the occasional interruption of blood flow in the double-lumen catheter and the occasional poor correlation between Biostator and reference glucose method, satisfactory glucose clamps could be maintained for 2 hours.

Key-Words

Glucose Clamp - Biostator - Artificial Endocrine Pancreas

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